无需分离的外泌体定量的基于适配体的荧光偏振测定

核心信息

• 论文英文标题: Aptamer-based fluorescence polarization assay for separation-free exosome quantification
• 期刊: Nanoscale
• 发表年份: 2019
• DOI: 10.1039/c9nr01589b
• PMID/PMCID: 31089660

研究概览

Tumor-derived exosomes have emerged as promising cancer biomarkers and attracted increasing interest in non-invasive cancer diagnosis and treatment monitoring. However, the identification and quantification of exosomes in clinical samples such as blood remains challenging due to the difficulty in trade-off between recognition specificity and isolation efficiency.

核心发现

Tumor-derived exosomes have emerged as promising cancer biomarkers and attracted increasing interest in non-invasive cancer diagnosis and treatment monitoring.

However, the identification and quantification of exosomes in clinical samples such as blood remains challenging due to the difficulty in trade-off between recognition specificity and isolation efficiency.

Here we have developed an aptamer-based fluorescence polarization assay for exosome quantification, which is a separation-free, amplification-free and sensitive approach enabling direct quantification of exosomes in human plasma.

While the key specificity of this assay is based on the aptamer's inherent affinity to membrane proteins on exosomes, exosomes' inherent huge mass/volume acts as mass-based fluorescence polarization amplifier.

Echo Biotech 角色

Echo Biotech's specific contribution should be verified from the full text or internal project record.

关联平台: To Be Verified

关联服务与能力: Co-authored Research

关联产品或试剂: 无明确产品引用

参考文献

原始论文: Aptamer-based fluorescence polarization assay for separation-free exosome quantification Nanoscale. 2019. DOI: 10.1039/c9nr01589b. PMID/PMCID: 31089660.