星形胶质细胞衍生EVs通过重塑视网膜微环境稳态减轻视神经损伤

核心信息

• 论文英文标题: Astrocyte-Derived Extracellular Vesicles Alleviate Optic Nerve Injury Through Remodeling of Retinal Microenvironmental Homeostasis
• 期刊: Invest Ophthalmol Vis Sci
• 发表年份: 2025
• DOI: 10.1167/iovs.66.4.16
• PMID/PMCID: 40192635; PMC11980952

研究概览

This study aimed to investigate whether ADEVs have a similar neuroprotective effect on RGCs in an optic nerve crush (ONC) rat model. Our findings provide a new cell-free therapeutic strategy for optic neuropathy.

核心发现

ADEVs protected RGCs from progressive loss and improved visual function.

ADEVs also significantly increased the expression of glial fibrillary acidic protein (GFAP) and modulated microglial activation.

The miRNAs associated with ADEVs were targeted by neuroprotective signals, such as MAPK, PI3K-AKT, and TNF-α, and through the targeting network generated via retinal mRNA sequencing, we found that potential functional genes, such as THBS1, PAK3, and Gstm1, likely participate in microenvironmental regulation.

研究设计

ADEVs were collected from primary rat astrocytes, and an ONC model was established to evaluate the effects of ADEVs on retinal structure and visual function using optical coherence tomography (OCT), hematoxylin and eosin (H&E) staining, and flash visual evoked potential (f-VEP) analysis.

Immunofluorescence was used to examine RGCs and investigate reactive gliotic changes.

Additionally, miRNA sequencing of ADEVs and retinal mRNA sequencing were performed to identify the potential mechanisms involved.

Echo Biotech 角色

细胞上清外泌体miRNA测序分析

关联平台: Exoomics®

关联服务与能力: EV miRNA Profiling / Sequencing

关联产品或试剂: 无明确产品引用

参考文献

原始论文: Astrocyte-Derived Extracellular Vesicles Alleviate Optic Nerve Injury Through Remodeling of Retinal Microenvironmental Homeostasis Investigative ophthalmology & visual science. 2025. DOI: 10.1167/iovs.66.4.16. PMID/PMCID: 40192635; PMC11980952.