CAF外泌体miR-21-5p抑制骨肉瘤中的铁死亡并促进增殖和迁移
核心信息
- 论文英文标题: CAFs exosomal miR-21-5p suppresses ferroptosis and promotes proliferation and migration in osteosarcoma
- 期刊: Cancer Cell Int
- 发表年份: 2025
- DOI: 10.1186/s12935-025-03930-8
- PMID/PMCID: 40826086; PMC12362868
研究概览
Osteosarcoma is one of the malignant tumors in children and adolescents patients. Our findings indicate that exosomal miR-21-5p could potentially serve as a therapeutic target in osteosarcoma, providing initial evidence for further clinical investigation.
核心发现
Single-cell RNA sequencing analysis of GSE162454 revealed the crucial role of CAFs in the pathogenesis of osteosarcoma.
Tissue exosomal miRNA sequencing unveiled significant differential expression of miR-21-5p.
Subsequently, aberrant upregulation of exosomal miR-21-5p exhibited a strong correlation with advanced clinical stage and poor prognosis in osteosarcoma.
Further experiments in vitro indicated that elevated levels of miR-21-5p significantly enhanced proliferation and migration of osteosarcoma cells by suppressing ferroptosis.
研究设计
Single-cell RNA sequencing was employed to characterize the microenvironment of osteosarcoma.
Tissue exosomal miRNA sequencing was conducted on tumor tissues and adjacent normal tissues to screen for abnormal expression of exosomal miRNAs.
WGCNA analysis was used to identify target exosomal miRNAs.
Echo Biotech 角色
骨肉瘤组织和正常组织外泌体分离表征&外泌体miRNA测序分析
关联平台: Exoomics®, Research Reagents & Tools
关联服务与能力: Tissue EV Isolation & Purification, EV Basic Characterization (TEM/NTA/WB), EV miRNA Profiling / Sequencing, Research Reagent / Product Supply
关联产品或试剂: Exosupur® EV Isolation/Purification Kit
参考文献
原始论文: CAFs exosomal miR-21-5p suppresses ferroptosis and promotes proliferation and migration in osteosarcoma Cancer cell international. 2025. DOI: 10.1186/s12935-025-03930-8. PMID/PMCID: 40826086; PMC12362868.