血浆神经源性EVs中的货物分子揭示了原发性开角型青光眼的病理特征

核心信息

  • 论文英文标题: Cargo molecules in plasma neural-derived extracellular vesicles reveal pathological signatures of primary open-angle glaucoma
  • 期刊: J Transl Med
  • 发表年份: 2026
  • DOI: 10.1186/s12967-026-08198-8
  • PMID/PMCID: 42063079; PMC13277049

研究概览

The aim of this study was to isolate NDEVs from the peripheral blood of patients with primary open-angle glaucoma (POAG) and investigate molecular alterations. This study provides a novel strategy for the discovery of noninvasive biomarkers for neurological diseases and elucidation of their potential molecular mechanisms.

核心发现

Immunoelectron microscopic analysis confirmed that GLAST proteins were localized in the EV membrane.

Tau and GFAP levels were significantly elevated in the NDEVs of patients with POAG but not in cell-free plasma or total EVs, indicating the superior diagnostic value of these parameters for POAG.

The target genes of DE-miRs in L1CAM⁺ EVs were biased towards neuronal functions, whereas those in GLAST⁺ EVs were biased towards glial inflammation.

In the retinas of COH mice, the expression of miR-140-3p and miR-183-5p was significantly upregulated, which was consistent with the results of the human plasma NDEV sequencing.

研究设计

NDEVs were immunoprecipitated using specific antibodies against L1CAM and GLAST and characterized using transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA), and western blotting.

ELISA was used to validate the enrichment of CNS markers and retina-specific proteins in the NDEV subtypes.

The diagnostic efficacy of tau and GFAP in cell-free plasma, total EVs, and NDEVs for POAG was compared via ELISAs.

Echo Biotech 角色

小鼠视网膜组织外泌体分离表征&蛋白组学(公司引用错了)&血浆神经源EV亚群miRNA测序分析

关联平台: Exoomics®, Research Reagents & Tools

关联服务与能力: Biofluid EV Isolation & Purification, Tissue EV Isolation & Purification, EV Basic Characterization (TEM/NTA/WB), EV miRNA Profiling / Sequencing, Research Reagent / Product Supply

关联产品或试剂: Exosupur® EV Isolation/Purification Kit

参考文献

原始论文: Cargo molecules in plasma neural-derived extracellular vesicles reveal pathological signatures of primary open-angle glaucoma Journal of Translational Medicine. 2026. DOI: 10.1186/s12967-026-08198-8. PMID/PMCID: 42063079; PMC13277049.