1型糖尿病血浆源性外泌体lncRNA表达谱

核心信息

  • 论文英文标题: Characterization of lncRNA Profiles of Plasma-Derived Exosomes From Type 1 Diabetes Mellitus
  • 期刊: FRONT ENDOCRINOL
  • 发表年份: 2022
  • DOI: 10.3389/fendo.2022.822221
  • PMID/PMCID: 35634499; PMC9135040

研究概览

In the present study, we characterized the plasma-derived exosomal lncRNAs expression profiles of T1DM and predict their potential function in the pathogenesis of T1DM. A better understanding of exosomal lncRNA profiling will provide novel insights into its molecular mechanisms.

核心发现

A total of 162 differentially expressed exosomal lncRNAs were identified in T1DM patients compared with control subjects, among which 77 up-regulated and 85 down-regulated.

The expression level of the selected six lncRNAs didn't show significant difference in the following qRT-PCR analysis.

Gene Ontology analysis enriched terms such as activation of phospholipase D activity, neuronal cell body membrane, and calcium sensitive guanylate cyclase activator activity for cis-acting genes of lncRNAs, and metal ion binding for trans-acting genes.

The most enriched Kyoto Encyclopedia of Genes and Genomes pathways for the lncRNAs were associated with oxidative phosphorylation and Parkinson's disease for cis-acting genes, and pathways in cancer as well as focal adhesion for trans-acting genes.

研究设计

Exosomal lncRNA expression profiles were detected by Illumina Hiseq platform (T1DM subjects N=10; age-, sex- matched Control subjects N=10).

Six exosomal lncRNAs were selected to validate their expression level by using quantitative real-time PCR (qRT-PCR) (T1DM subjects N=30; age-, sex- matched Control subjects N=30).

Bioinformatics analysis approaches were carried out to explore the potential biological function of differentially expressed lncRNAs.

Echo Biotech 角色

外泌体长链测序+qPCR验证--与EA56、EA61同一个长链数据拆分发表

关联平台: Exoomics®, Research Reagents & Tools

关联服务与能力: EV Isolation & Purification, EV lncRNA/mRNA Sequencing, qPCR Validation, Research Reagent / Product Supply

关联产品或试剂: Exosupur® EV Isolation/Purification Kit

参考文献

原始论文: Characterization of lncRNA Profiles of Plasma-Derived Exosomes From Type 1 Diabetes Mellitus Frontiers in Endocrinology. 2022. DOI: 10.3389/fendo.2022.822221. PMID/PMCID: 35634499; PMC9135040.