循环血浆EVs RNA图谱检测T1a期结直肠癌和癌前晚期腺瘤
核心信息
- 论文英文标题: Circulating small extracellular vesicle RNA profiling for the detection of T1a stage colorectal cancer and precancerous advanced adenoma
- 期刊: Elife
- 发表年份: 2024
- DOI: 10.7554/eLife.88675
- PMID/PMCID: 39121006; PMC11315448
研究概览
Here, we aim to enable liquid biopsy of T1a stage colorectal cancer (CRC) and precancerous advanced adenoma (AA) by profiling circulating small extracellular vesicle (sEV)-derived RNAs. Then we further categorized these sEV-RNAs into six modules by a weighted gene coexpression network analysis and constructed a 60-gene t-SNE model consisting of the top 10 RNAs of each module that could well distinguish T1a stage CRC/AA from NC samples.
核心发现
It takes more than 20 years for normal colorectal mucosa to develop into metastatic carcinoma.
The long time window provides a golden opportunity for early detection to terminate the malignant progression.
Here, we aim to enable liquid biopsy of T1a stage colorectal cancer (CRC) and precancerous advanced adenoma (AA) by profiling circulating small extracellular vesicle (sEV)-derived RNAs.
We exhibited a full RNA landscape for the circulating sEVs isolated from 60 participants.
Echo Biotech 角色
血浆外泌体分离鉴定&全转录组测序分析&qPCR验证
关联平台: Exoomics®
关联服务与能力: Biofluid EV Isolation & Purification, EV Basic Characterization (TEM/NTA/WB), EV Whole-Transcriptome Sequencing, qPCR Validation
关联产品或试剂: 无明确产品引用
参考文献
原始论文: Circulating small extracellular vesicle RNA profiling for the detection of T1a stage colorectal cancer and precancerous advanced adenoma Elife. 2024. DOI: 10.7554/eLife.88675. PMID/PMCID: 39121006; PMC11315448.