循环sEV miRNA和CA-125对晚期卵巢癌患者进行治疗分层-术前识别可R0切除患者
核心信息
- 论文英文标题: Circulating small extracellular vesicles microRNAs plus CA-125 for treatment stratification in advanced ovarian cancer
- 期刊: J Transl Med
- 发表年份: 2023
- DOI: 10.1186/s12967-023-04774-4
- PMID/PMCID: 38129848; PMC10740240
研究概览
Using a comprehensive plasma small extracellular vesicles (sEVs) microRNAs (miRNAs) profile in AOC, we identified and optimized a risk prediction model consisting of plasma sEVs-derived 4-miRNA and CA-125 with better performance in predicting R0 resection. There is an unmet clinical need for selecting primary or interval debulking surgery in AOC patients using existing prediction models.
核心发现
Using a comprehensive plasma small extracellular vesicles (sEVs) microRNAs (miRNAs) profile in AOC, we identified and optimized a risk prediction model consisting of plasma sEVs-derived 4-miRNA and CA-125 with better performance in predicting R0 resection.
研究设计
RNA sequencing of circulating small extracellular vesicles (sEVs) was used to discover the differential expression microRNAs (DEMs) profile between any residual disease (R0, n = 17) and no residual disease (non-R0, n = 20) in AOC patients.
We further analyzed plasma samples of AOC patients collected before surgery or neoadjuvant chemotherapy via TaqMan qRT-PCR.
The combined risk model of residual disease was developed by logistic regression analysis based on the discovery-validation sets.
Echo Biotech 角色
血浆大小囊泡分离鉴定 及 血浆外泌体qPCR验证+酶消化验证 及 组织外泌体分离鉴定+酶消化验证+不同细胞类型marker蛋白WB检测
关联平台: Exoomics®, Research Reagents & Tools
关联服务与能力: Biofluid EV Isolation & Purification, Tissue EV Isolation & Purification, EV Basic Characterization (TEM/NTA/WB), qPCR Validation, Research Reagent / Product Supply
关联产品或试剂: Exosupur® EV Isolation/Purification Kit
参考文献
原始论文: Circulating small extracellular vesicles microRNAs plus CA-125 for treatment stratification in advanced ovarian cancer J Transl Med. 2023. DOI: 10.1186/s12967-023-04774-4. PMID/PMCID: 38129848; PMC10740240.