尺寸排阻色谱和离子交换吸附相结合改进血浆EVs蛋白质组学分析

核心信息

• 论文英文标题: Combination of Size-Exclusion Chromatography and Ion Exchange Adsorption for Improving the Proteomic Analysis of Plasma-derived Extracellular Vesicles
• 期刊: Proteomics
• 发表年份: 2023
• DOI: 10.1002/pmic.202200364
• PMID/PMCID: 36624553

研究概览

In this study, two EV isolation strategies, size exclusion chromatography (SEC) and SEC combined with ion exchange adsorption (SEC + IEA), were compared in terms of the purity and quantity of protein in EVs. Our results demonstrated that, compared to single-step SEC, SEC combined with IEA could produce plasma-derived EVs with a higher purity by decreasing the abundance of lipoprotein.

核心发现

Extracellular vesicles (EVs) are lipid membrane vesicles released by live cells that carry a variety of biomolecules, including nucleic acids, lipids, and proteins.

Recently, proteins in plasma-derived EVs have emerged as novel biomarkers with essential functions in the diagnosis and prognosis of human diseases.

However, the current methods of isolating EVs from plasma often lead to coisolated impurities in biological fluids.

Therefore, before performing any research protocol, the process of extracting EVs from plasma for proteomic analysis must be optimized.

Echo Biotech 角色

Echo Biotech contributed EV isolation and purification; the study also used or cited Exosupur®.

关联平台: Exoomics®, Research Reagents & Tools

关联服务与能力: EV Isolation & Purification, Research Reagent / Product Supply

关联产品或试剂: Exosupur® EV Isolation/Purification Kit

参考文献

原始论文: Combination of Size-Exclusion Chromatography and Ion Exchange Adsorption for Improving the Proteomic Analysis of Plasma-derived Extracellular Vesicles Proteomics. 2023. DOI: 10.1002/pmic.202200364. PMID/PMCID: 36624553.