体积排阻色谱法和超速离心法的结合改善了血浆衍生的小细胞外囊泡的蛋白质组学分析

核心信息

• 论文英文标题: Combination of Size-Exclusion Chromatography and Ultracentrifugation Improves the Proteomic Profiling of Plasma-Derived Small Extracellular Vesicles
• 期刊: Biol Proced Online
• 发表年份: 2020
• DOI: 10.1186/s12575-020-00125-5
• PMID/PMCID: 32587481; PMC7313174

研究概览

Circulating small extracellular vesicles (sEVs) and its associated proteins are of great interest in the early detection of many diseases. Combining SEC with UC could significantly improve the performance of mass spectrometry-based proteomic profiling in analyzing plasma-derived sEVs.

核心发现

Plasma samples were collected from healthy individuals, and sEVs were separated by one-step SEC, one-step UC, and combining SEC with UC, respectively.

Here we exhibited that the purity of sEVs was improved by SEC in contrast to traditional UC.

Furthermore, by conducting a SEC procedure followed by UC, we separated sEVs with the highest purity.

In the proteomic analysis, 992 protein species were identified in the plasma sEVs isolated by our novel separation method, of which several proteins are sEVs-associated proteins but hitherto never been identified in the previous studies and database, much more than plasma sEVs isolated by UC (453) or SEC (682) alone.

Echo Biotech 角色

Echo Biotech contributed EV isolation and purification; the study also used or cited Exosupur®.

关联平台: Exoomics®, Research Reagents & Tools

关联服务与能力: EV Isolation & Purification, Research Reagent / Product Supply

关联产品或试剂: Exosupur® EV Isolation/Purification Kit

参考文献

原始论文: Combination of Size-Exclusion Chromatography and Ultracentrifugation Improves the Proteomic Profiling of Plasma-Derived Small Extracellular Vesicles Biol Proced Online. 2020. DOI: 10.1186/s12575-020-00125-5. PMID/PMCID: 32587481; PMC7313174.