川芎嗪与小檗碱联合应用通过调控血小板外泌体miR-34a-5p预防大鼠冠状动脉微栓塞
核心信息
- 论文英文标题: A combination of tetramethylpyrazine and berberine prevents coronary microembolization in rats by regulating miR-34a-5p in platelet-derived exosomes
- 期刊: J Ethnopharmacol
- 发表年份: 2025
- DOI: 10.1016/j.jep.2025.120549
- PMID/PMCID: 40915375
研究概览
This study was designed to explore the potential role and mechanisms of TMP combined with BBR in reducing the formation of coronary microembolization (CME) in rats. Chuanxiong rhizome and Coptis chinensis are a classic herbal pair in Traditional Chinese Medicine (TCM), renowned for their effects in activating blood circulation and resolving toxicity.
核心发现
The combination of TMP and BBR significantly mitigated CME formation, myocardial injury, and left ventricular ejection fraction (LVEF) in rats.
Besides, the pretreatment with TMP and BBR inhibited platelet activation, alleviated endothelial cell damage, and reduced the number of M1 macrophages.
Additionally, the levels of miR-34a-5p in PLT-exo of CME rats were suppressed after the pretreatment with TMP and BBR.
However, these effects of TMP and BBR were attenuated after the injection of agomiR-34a-5p in CME rats.
研究设计
CME was induced by the injection of sodium laurate into the left ventricular of rats.
CME formation and myocardial damage were examined using histopathology, while echocardiography was used to assess cardiac functions.
Moreover, TMP and BBR were added to observe their effects.
Echo Biotech 角色
富血小板血浆PRP外泌体分离+qPCR检测分析
关联平台: Exoomics®
关联服务与能力: Biofluid EV Isolation & Purification, qPCR Validation
关联产品或试剂: 无明确产品引用
参考文献
原始论文: A combination of tetramethylpyrazine and berberine prevents coronary microembolization in rats by regulating miR-34a-5p in platelet-derived exosomes Journal of ethnopharmacology. 2025. DOI: 10.1016/j.jep.2025.120549. PMID/PMCID: 40915375.