人脐带间充质干细胞及其小细胞外囊泡治疗骨关节炎的疗效比较
核心信息
- 论文英文标题: Comparison of Curative Effect of Human Umbilical Cord-Derived Mesenchymal Stem Cells and Their Small Extracellular Vesicles in Treating Osteoarthritis
- 期刊: INT J NANOMED
- 发表年份: 2021
- DOI: 10.2147/IJN.S336062
- PMID/PMCID: 34938076; PMC8687685
研究概览
This study aimed to compare the therapeutic effects of two agents on osteoarthritis (OA) and investigate underlying mechanism using proteomics. These data suggest the promising potential of hUC-MSC-sEVs as a therapeutic agent for OA.
核心发现
In vitro data showed that hUC-MSC-sEVs were taken up by chondrocytes.
A total of 15 μg/mL of sEVs show the greatest proliferative and migratory capacities among all groups.
In the animal study, hUC-MSCs and hUC-MSC-sEVs alleviated cartilage damage.
This effect was mediated via maintaining cartilage homeostasis, as was confirmed by upregulation of the COL II and downregulation of the MMP13 and ADAMTS5.
研究设计
In vitro, the proliferation and migration abilities of chondrocytes treated with hUC-MSCs or hUC-MSC-sEVs were detected by Cell Counting Kit-8 assay and scratch wound assay.
In vivo, hUC-MSCs (a single dose of 5 × 105) or hUC-MSC-sEVs (30 μg/time) were injected into the knee joints of anterior cruciate ligament transection-induced OA model.
Hematoxylin and eosin, Safranin O/Fast Green staining were used to observe cartilage degeneration.
Echo Biotech 角色
Echo Biotech contributed EV isolation and purification; the study also used or cited Exosupur®.
关联平台: Exoomics®, Research Reagents & Tools
关联服务与能力: EV Isolation & Purification, Research Reagent / Product Supply
关联产品或试剂: Exosupur® EV Isolation/Purification Kit
参考文献
原始论文: Comparison of Curative Effect of Human Umbilical Cord-Derived Mesenchymal Stem Cells and Their Small Extracellular Vesicles in Treating Osteoarthritis INT J NANOMED. 2021. DOI: 10.2147/IJN.S336062. PMID/PMCID: 34938076; PMC8687685.