基于低温恒温器冷冻切片方法提高不同组织外泌体分离产量

核心信息

• 论文英文标题: A cryostat-based frozen section method to increase the yield of extracellular vesicles extracted from different tissues
• 期刊: Biotechniques
• 发表年份: 2022
• DOI: 10.2144/btn-2022-0035
• PMID/PMCID: 35946315

研究概览

Extracellular vesicles (EVs) are small vesicles mediating intercellular communications that have been widely used in disease diagnosis. Extracting EVs from tissues is of great importance, but current approaches are finite and the EV yield is limited.

核心发现

Extracellular vesicles (EVs) are small vesicles mediating intercellular communications that have been widely used in disease diagnosis.

Extracting EVs from tissues is of great importance, but current approaches are finite and the EV yield is limited.

Here, the authors introduced a new method to increase EV yield based on frozen sectioning.

With a standardized, semiautomated tissue-slicing procedure in a cryostat, the authors successfully isolated EVs from hearts, kidneys and stomachs.

Echo Biotech 角色

Echo Biotech's specific contribution should be verified from the full text or internal project record.

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参考文献

原始论文: A cryostat-based frozen section method to increase the yield of extracellular vesicles extracted from different tissues Biotechniques. 2022. DOI: 10.2144/btn-2022-0035. PMID/PMCID: 35946315.