通过EVs传递miR-23b-3p以促进细胞衰老和异常脂质代谢
核心信息
- 论文英文标题: Delivering miR-23b-3p by small extracellular vesicles to promote cell senescence and aberrant lipid metabolism
- 期刊: BMC Biol
- 发表年份: 2025
- DOI: 10.1186/s12915-025-02143-9
- PMID/PMCID: 39934790; PMC11817603
研究概览
In the present study, we extracted liver tissue small extracellular vesicles (sEVs) of mice at different ages and performed transcriptome and proteome analyses to investigate the senescence-associated secretory phenotype (SASP) and mechanisms. Targeting miR-23b-3p could provide new therapeutic strategies for ameliorating age-related liver dysfunction and associated metabolic abnormalities.
核心发现
In the present study, we extracted liver tissue small extracellular vesicles (sEVs) of mice at different ages and performed transcriptome and proteome analyses to investigate the senescence-associated secretory phenotype (SASP) and mechanisms.
sEVs in the older group were rich in miR-23b-3p, which was abundant in the sEVs of induced aging cells and promoted cell senescence by targeting TNF alpha induced protein 3 (Tnfaip3).
After injecting adeno-associated virus (AAV) expressing miR-23b-3p into mice, the liver of mice in the experimental group displayed a more evident inflammatory response than that in the control group.
Additionally, we found elevated miR-23b-3p in blood-derived-sEVs from patients with familial hypercholesterolemia.
Echo Biotech 角色
肝脏组织外泌体分离表征&miRNA测序分析&LFQ蛋白质组学&qPCR验证
关联平台: Exoomics®, Research Reagents & Tools
关联服务与能力: Biofluid EV Isolation & Purification, Tissue EV Isolation & Purification, EV Basic Characterization (TEM/NTA/WB), EV miRNA Profiling / Sequencing, EV Proteomics, qPCR Validation, Research Reagent / Product Supply
关联产品或试剂: Exosupur® EV Isolation/Purification Kit
参考文献
原始论文: Delivering miR-23b-3p by small extracellular vesicles to promote cell senescence and aberrant lipid metabolism BMC biology. 2025. DOI: 10.1186/s12915-025-02143-9. PMID/PMCID: 39934790; PMC11817603.