通过基于无需标准化qPCR 的循环细胞外囊泡 RNA 特征检测胰腺导管腺癌

核心信息

• 论文英文标题: Detection of Pancreatic Ductal Adenocarcinoma by A qPCR-based Normalizer-free Circulating Extracellular Vesicles RNA Signature
• 期刊: J Cancer
• 发表年份: 2021
• DOI: 10.7150/jca.50716
• PMID/PMCID: 33531989; PMC7847660

研究概览

Background: Pancreatic ductal adenocarcinoma (PDAC) is difficult to diagnose and many efforts have been made to evaluate EVs-derived RNAs as biomarkers to predict PDAC. Conclusion: We've developed a qPCR-based, normalizer-free circulating EVs RNA classifier, which could well distinguish PDAC patients from noncancerous controls.

核心发现

Background: Pancreatic ductal adenocarcinoma (PDAC) is difficult to diagnose and many efforts have been made to evaluate EVs-derived RNAs as biomarkers to predict PDAC.

However, lack of robust internal references largely limited their clinical application.

Here we proposed an RNA ratio-based, normalizer-free algorithm to quantitate EVs-derived RNAs in PDAC.

Methods: Differentially expressed RNAs in the training group were identified using "limma" package.

Echo Biotech 角色

Echo Biotech's specific contribution should be verified from the full text or internal project record.

关联平台: To Be Verified

关联服务与能力: Role Not Identified

关联产品或试剂: 无明确产品引用

参考文献

原始论文: Detection of Pancreatic Ductal Adenocarcinoma by A qPCR-based Normalizer-free Circulating Extracellular Vesicles RNA Signature J Cancer. 2021. DOI: 10.7150/jca.50716. PMID/PMCID: 33531989; PMC7847660.