裂解液或组织-80°C储存对随后提取的上皮卵巢癌组织EVs的影响

核心信息

• 论文英文标题: Effects of lysate/tissue storage at -80° C on subsequently extracted EVs of epithelial ovarian cancer tissue origins
• 期刊: iScience
• 发表年份: 2023
• DOI: 10.1016/j.isci.2023.106521
• PMID/PMCID: 37123245; PMC10139970

研究概览

Small extracellular vesicles (sEVs) and large extracellular vesicles (lEVs), play vital roles in intercellular communication. Cryopreserved EOC tissue may benefit clinical applications for studies of tissue-derived EVs, especially EV proteins-related ones.

核心发现

Small extracellular vesicles (sEVs) and large extracellular vesicles (lEVs), play vital roles in intercellular communication.

We optimized a method that extracts EVs from epithelial ovarian cancer (EOC) tissues for the purpose of investigating whether cryopreservation of EOC tissues affects the phenotypes, contents, and biological functions of extracted EVs.

EV morphology, the number and size distribution of EVs, and EV-related markers were analyzed.

Storage of lysates at -80°C decreased lEV yield and increased sEV yield, whereas storage of tissues at -80°C increased both sEV and lEV yields; neither changed the morphology or particle mass ratio of EVs.

Echo Biotech 角色

组织裂解上清大小囊泡分离鉴定及miRNA测序分析&蛋白质组学

关联平台: Exoomics®

关联服务与能力: Tissue EV Isolation & Purification, EV Basic Characterization (TEM/NTA/WB), EV miRNA Profiling / Sequencing, EV Proteomics

关联产品或试剂: 无明确产品引用

参考文献

原始论文: Effects of lysate/tissue storage at -80° C on subsequently extracted EVs of epithelial ovarian cancer tissue origins iScience. 2023. DOI: 10.1016/j.isci.2023.106521. PMID/PMCID: 37123245; PMC10139970.