工程化EVs作为溶酶体降解PCSK9的纳米海绵
核心信息
- 论文英文标题: Engineered extracellular vesicles as nanosponges for lysosomal degradation of PCSK9
- 期刊: Mol Ther
- 发表年份: 2024
- DOI: 10.1016/j.ymthe.2024.11.034
- PMID/PMCID: 39604267; PMC11853006
研究概览
In this study, extracellular vesicles (EVs) were engineered to nanosponges, which could efficiently adsorb and deliver PCSK9 into lysosomes for degradation. In this study, extracellular vesicles (EVs) were engineered to nanosponges, which could efficiently adsorb and deliver PCSK9 into lysosomes for degradation.
核心发现
Proprotein convertase subtilisin/kexin type 9 (PCSK9) plays a crucial role in the degradation of the low-density lipoprotein receptor (LDLR), and PCSK9 inhibition emerges as an attractive strategy for atherosclerosis management.
In this study, extracellular vesicles (EVs) were engineered to nanosponges, which could efficiently adsorb and deliver PCSK9 into lysosomes for degradation.
Briefly, nanosponges were engineered by modifying EVs with EGF-A/PTGFRN fusion protein (PCSK9 binding domain EGF-A from the mutant LDLR with higher affinity was fused to the C terminus of prostaglandin F2 receptor negative regulator).
The modification endowed the EVs with hundreds of EGF-As displayed on the surface, and thus the capacity to adsorb PCSK9 efficiently.
Echo Biotech 角色
Exosupur ES850+ES911 (293F EVs膜外负载蛋白作为纳米海绵吸附并将PCSK9递送至溶酶体降解)
关联平台: Exoomics®, Echosome®, Research Reagents & Tools
关联服务与能力: Cell-Culture Supernatant EV Isolation & Purification, Small RNA / Cargo Loading, Research Reagent / Product Supply
关联产品或试剂: Exosupur® EV Isolation/Purification Kit
参考文献
原始论文: Engineered extracellular vesicles as nanosponges for lysosomal degradation of PCSK9 Molecular therapy. 2024. DOI: 10.1016/j.ymthe.2024.11.034. PMID/PMCID: 39604267; PMC11853006.