循环小sEV miRNAs作为早期肺癌不同病理类型的诊断生物标志物的评价-SCLC和NSCLC和健康人，血浆sEV miRNA测序+小样本量qPCR验证

核心信息

论文英文标题: Evaluation of circulating small extracellular vesicle-derived miRNAs as diagnostic biomarkers for differentiating between different pathological types of early lung cancer
期刊: SCI REP-UK
发表年份: 2022
DOI: 10.1038/s41598-022-22194-0
PMID/PMCID: 36229645; PMC9561663

研究概览

The present study was designed to explore whether plasma-derived sEV miRNAs could be utilized as diagnostic biomarkers for differentiating between early-stage small cell lung cancer (SCLC) and early-stage non-small cell lung cancer (NSCLC). We selected six representative DEMs for validation by qRT‒PCR, which confirmed that miRNA-483-3p derived from plasma sEVs could be used as a potential biomarker for the diagnosis of early-stage SCLC, miRNA-152-3p and miRNA-1277-5p could be used for the diagnosis of early-stage NSCLC respectively.

核心发现

Lung cancer is the leading cause of cancer-related death worldwide.

MicroRNAs (miRNAs) in circulating small extracellular vesicles (sEVs) have been suggested to be potential biomarkers for cancer diagnosis.

We compared the miRNA profiles of plasma-derived sEVs from healthy individuals, patients with early-stage SCLC and patients with early-stage NSCLC.

Echo Biotech 角色

Echo Biotech contributed EV isolation and purification; the study also used or cited Exosupur®.

关联平台: Exoomics®, Research Reagents & Tools

关联服务与能力: EV Isolation & Purification, Research Reagent / Product Supply

关联产品或试剂: Exosupur® EV Isolation/Purification Kit

参考文献

原始论文: Evaluation of circulating small extracellular vesicle-derived miRNAs as diagnostic biomarkers for differentiating between different pathological types of early lung cancer Scientific reports. 2022. DOI: 10.1038/s41598-022-22194-0. PMID/PMCID: 36229645; PMC9561663.