循环小细胞外囊泡衍生的 miRNA 作为早期结肠癌生物标志物的评估：与血浆总 miRNA 头对头比较
核心信息
- 论文英文标题: Evaluation of circulating small extracellular vesicles derived miRNAs as biomarkers of early colon cancer: a comparison with plasma total miRNAs
- 期刊: J Extracell Vesicles
- 发表年份: 2019
- DOI: 10.1080/20013078.2019.1643670
- PMID/PMCID: 31448068; PMC6691764
研究概览
Early diagnosis of colon cancer (CC) is clinically important, as it can significantly improve patients' survival rate and quality of life. In conclusion, we suggested that circulating sEVs enriched fractions have a distinct miRNA profile in CC patients, and sEVs derived miRNA could be used as a promising biomarker to detect CC at an early stage.
核心发现
Early diagnosis of colon cancer (CC) is clinically important, as it can significantly improve patients' survival rate and quality of life.
Although the potential role for small extracellular vesicles (sEVs) in early detection of many diseases has been repeatedly mentioned, systematic screening of plasma sEVs derived early CC specific biomarkers has not yet been reported.
In this work, plasma sEVs enriched fractions were derived from 15 early-stage (TisN0M0) CC patients and 10 normal controls (NC).
RNA sequencing identified a total number of 95 sEVs enriched fraction derived miRNAs with differential expression between CC and NC, most of which (60/95) was in well accordance with tissue results in the Cancer Genome Atlas (TCGA) dataset.
Echo Biotech 角色
血浆外泌体分离鉴定&miRNA测序分析&qPCR验证
关联平台: Exoomics®
关联服务与能力: Biofluid EV Isolation & Purification, EV Basic Characterization (TEM/NTA/WB), EV miRNA Profiling / Sequencing, qPCR Validation
关联产品或试剂: 无明确产品引用
参考文献
原始论文: Evaluation of circulating small extracellular vesicles derived miRNAs as biomarkers of early colon cancer: a comparison with plasma total miRNAs J Extracell Vesicles. 2019. DOI: 10.1080/20013078.2019.1643670. PMID/PMCID: 31448068; PMC6691764.