血浆外泌体miRNA作为甲状腺乳头状癌淋巴结转移潜在诊断生物标志物的评估
核心信息
- 论文英文标题: Evaluation of plasma exosomal miRNAs as potential diagnostic biomarkers of lymph node metastasis in papillary thyroid carcinoma
- 期刊: Endocrine
- 发表年份: 2021
- DOI: 10.1007/s12020-021-02949-x
- PMID/PMCID: 34854020
研究概览
In the present study, we aimed to identify whether plasma exosomal miRNAs could be potential diagnostic markers of LNM in PTC. The two plasma exosomal miRNAs (miR-6774-3p and miR-6879-5p) and their combination could serve as new promising biomarkers for the diagnosis of LNM in PTC patients.
核心发现
In total, 197 miRNAs were found to be differentially expressed in the testing set.
001) in the PTC-N1 patients was significantly higher than that in the controls.
962) were higher than those of the total miRNAs directly isolated from plasma.
Moreover, the expression of exosomal miRNAs was stable after treatment with RNase A, prolonged incubation, or repeated freezing and thawing.
研究设计
Profiles of plasma exosomal miRNAs were screened using miRNA microarrays.
Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was performed in the validation and diagnostic sets to select candidate exosomal miRNAs.
Finally, receiver operating characteristic (ROC) curves were generated to evaluate the efficiency of target exosomal miRNAs in distinguishing PTC-N1 patients from PTC-N0 patients.
Echo Biotech 角色
外泌体电镜&粒径&WB鉴定
关联平台: Exoomics®
关联服务与能力: EV Basic Characterization (TEM/NTA/WB)
关联产品或试剂: 无明确产品引用
参考文献
原始论文: Evaluation of plasma exosomal miRNAs as potential diagnostic biomarkers of lymph node metastasis in papillary thyroid carcinoma Endocrine. 2021. DOI: 10.1007/s12020-021-02949-x. PMID/PMCID: 34854020.