内皮细胞外泌体 hsa-miR-299-3p 通过 JAK2/STAT5 磷酸化通路介导慢性肾病小鼠高磷诱导的血管钙化
核心信息
- 论文英文标题: Exosomal hsa-miR-299-3p from endothelium mediates high phosphorus-induced vascular calcification in mice model of CKD via phosphorylated JAK2/STAT5 pathway
- 期刊: Front Pharmacol
- 发表年份: 2026
- DOI: 10.3389/fphar.2026.1752954
- PMID/PMCID: 42038288; PMC13103977
研究概览
This study investigates the role of endothelial cells (ECs)-derived exosomal microRNAs in mediating osteogenic differentiation of vascular smooth muscle cells (VSMCs) under high phosphate (HP) conditions. The miR-299-3p/MARCH3/p-JAK2/STAT5 signaling axis represents a critical regulatory pathway in VC pathogenesis and offers a potential therapeutic target for this life-threatening complication of CKD.
核心发现
HP-stimulated ECs released Exos (HP-Exos) that were internalized by VSMCs and significantly promoted VC in both in vitro and in vivo models.
miRNA sequencing identified miR-299-3p as significantly upregulated in HP-Exos.
Functional studies demonstrated that exosomal miR-299-3p directly targeted membrane-associated RING-CH3 (MARCH3), leading to activation of the p-JAK2/STAT5 signaling pathway.
This cascade subsequently upregulated osteogenic markers and downregulated contractile marker, thereby promoting osteogenic differentiation of VSMCs.
研究设计
A CKD-VC mouse model was established using a HP and high-adenine diet.
Exosomes (Exos) were isolated from ECs cultured under normal or HP conditions.
The effects of Exos on calcification of VSMCs were evaluated using in vitro co-culture systems and in vivo administration.
Echo Biotech 角色
外泌体分离表征 & miRNA测序分析
关联平台: Exoomics®
关联服务与能力: EV Isolation & Purification, EV Basic Characterization (TEM/NTA/WB), EV miRNA Profiling / Sequencing
关联产品或试剂: 无明确产品引用
参考文献
原始论文: Exosomal hsa-miR-299-3p from endothelium mediates high phosphorus-induced vascular calcification in mice model of CKD via phosphorylated JAK2/STAT5 pathway Frontiers in pharmacology. 2026. DOI: 10.3389/fphar.2026.1752954. PMID/PMCID: 42038288; PMC13103977.