外泌体miR-224-3p通过靶向胃癌GSK3B促进淋巴管生成和淋巴结转移

核心信息

• 论文英文标题: Exosomal miR-224-3p promotes lymphangiogenesis and lymph node metastasis by targeting GSK3B in gastric cancer
• 期刊: Oncol Res
• 发表年份: 2025
• DOI: 10.32604/or.2024.050431
• PMID/PMCID: 39866224; PMC11753999

研究概览

In this study, we screened out miR-224-3p related to GC progression and LNM in a vascular endothelial growth Factor C (VEGFC)-independent manner. Our discovery of the exo-miR-224-3p/GSK3B/β-catenin/PROX1 axis may provide a new direction for the clinical treatment of GC.

核心发现

In this study, we screened out miR-224-3p related to GC progression and LNM in a vascular endothelial growth Factor C (VEGFC)-independent manner.

We found that exo-miR-224-3p derived from GC cells could enter human lymphatic endothelial cells (HLECs) and promote the tube formation and migration of HLECs.

In addition, it was revealed that miR-224-3p could bind to the 3'UTR region of GSK3B mRNA.

Then, we proved that inhibiting the expression of GSK3B could suppress the phosphorylation of β-catenin and promote the transcription of PROX1, thus leading to tumor lymphangiogenesis.

研究设计

We used sequencing analysis of collected serum to screen out exo-miRNA related to LNM in GC.

ELISA, qRT-PCR, Western Blot, RNA pull-down assay, Transwell assay, animal experiments, and other experiments were used to verify the results.

Echo Biotech 角色

nanoFCM粒径检测

关联平台: Exoomics®

关联服务与能力: EV Basic Characterization (TEM/NTA/WB)

关联产品或试剂: 无明确产品引用

参考文献

原始论文: Exosomal miR-224-3p promotes lymphangiogenesis and lymph node metastasis by targeting GSK3B in gastric cancer Oncology research. 2025. DOI: 10.32604/or.2024.050431. PMID/PMCID: 39866224; PMC11753999.