增殖性糖尿病视网膜病变PDR液体活检之玻璃体外泌体miRNA谱
核心信息
- 论文英文标题: Exosomal miRNA Profiling in Liquid Biopsy of Vitreous in Proliferative Diabetic Retinopathy
- 期刊: Invest Ophthalmol Vis Sci
- 发表年份: 2025
- DOI: 10.1167/iovs.66.9.24
- PMID/PMCID: 40637511; PMC12255188
研究概览
This study profiled exosomal microRNAs (exo-miRNAs) in the vitreous humor (VH) of patients with PDR to elucidate their regulatory roles in PDR pathogenesis. These findings indicate that vitreous exo-miRNAs, specifically miR-204-5p and miR-486-5p, represent promising diagnostic biomarkers and potential therapeutic targets for PDR.
核心发现
Exosomes were isolated and characterized from VH samples.
Sequencing analysis identified 843 unique miRNAs, with 60 showing significant differential expression between PDR and control groups (28 upregulated and 32 downregulated).
The miR-451a and miR-486-5p were most upregulated; miR-204-5p and miR-211-5p were most downregulated.
Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses revealed DEM target genes enriched in endocrine, metabolic, signaling transduction, and intercellular adhesion pathways, notably cGMP-PKG, mTOR, and cAMP pathways.
研究设计
VH samples were obtained from 23 patients with PDR and 17 non-diabetic controls.
Exosomes were isolated using ultracentrifugation and characterized via transmission electron microscopy, nanoparticle tracking analysis and Western blot.
High-throughput sequencing identified differentially expressed miRNAs (DEMs), followed by target gene prediction and bioinformatic analyses.
Echo Biotech 角色
玻璃体外泌体分离鉴定&miRNA测序分析和qPCR验证
关联平台: Exoomics®
关联服务与能力: Biofluid EV Isolation & Purification, EV Basic Characterization (TEM/NTA/WB), EV miRNA Profiling / Sequencing, qPCR Validation
关联产品或试剂: 无明确产品引用
参考文献
原始论文: Exosomal miRNA Profiling in Liquid Biopsy of Vitreous in Proliferative Diabetic Retinopathy Investigative ophthalmology & visual science. 2025. DOI: 10.1167/iovs.66.9.24. PMID/PMCID: 40637511; PMC12255188.