癌症相关成纤维细胞CAF外泌体POSTN驱动微浸润性肺腺癌的进展：单细胞和组织外泌体测序分析的见解

核心信息

  • 论文英文标题: Exosomal POSTN from cancer-associated fibroblasts drives progression of microinvasive lung adenocarcinoma: insights from single-cell and tissue exosome sequencing analysis
  • 期刊: Front Immunol
  • 发表年份: 2026
  • DOI: 10.3389/fimmu.2026.1767771
  • PMID/PMCID: 42238572; PMC13226168

研究概览

Integrated analyses of scRNA-seq data, exosomal transcriptomes, the TCGA-LUAD cohort, and an independent LUAD tissue/serum cohort were used to characterize POSTN expression and to evaluate its prognostic and diagnostic relevance. Exosomal POSTN derived from POSTN+ CAFs may represent an important stromal mediator of MIA/LUAD progression and a potential diagnostic and prognostic biomarker in early-stage LUAD.

核心发现

scRNA-seq identified a malignant Cancer-alveolar type II (Cancer-AT2) epithelial subset and multiple CAF subsets.

Among these, POSTN+ CAFs were enriched in MIA tissues and showed enhanced crosstalk with Cancer-AT2 cells through extracellular matrix (ECM)-related ligand-receptor interactions.

Tissue-derived exosomes contained 588 differentially expressed mRNAs, among which POSTN was markedly upregulated and showed the strongest association with fibroblast-related signatures.

POSTN was predominantly expressed in fibroblasts across independent non-small cell lung cancer datasets and was elevated in LUAD tissues, tissue-derived exosomes, and serum exosomes, correlating with advanced stage, lymph node metastasis, and poor survival.

研究设计

Single-cell RNA sequencing (scRNA-seq) and tissue-derived exosomal RNA sequencing were performed on four primary MIA lesions and matched adjacent lung tissues.

Integrated analyses of scRNA-seq data, exosomal transcriptomes, the TCGA-LUAD cohort, and an independent LUAD tissue/serum cohort were used to characterize POSTN expression and to evaluate its prognostic and diagnostic relevance.

Primary MIA-associated POSTN+ and POSTN- CAFs were isolated for exosome preparation, followed by co-culture experiments with LUAD cell lines and xenograft assays.

Echo Biotech 角色

8个样本单细胞转录组测序&组织外泌体长链RNA测序分析&联合分析&组织外泌体分离鉴定

关联平台: Exoomics®

关联服务与能力: Tissue EV Isolation & Purification, EV Basic Characterization (TEM/NTA/WB), EV lncRNA/mRNA Sequencing, EV Whole-Transcriptome Sequencing, Single-Cell RNA Sequencing, Integrated Multi-omics Analysis

关联产品或试剂: 无明确产品引用

参考文献

原始论文: Exosomal POSTN from cancer-associated fibroblasts drives progression of microinvasive lung adenocarcinoma: insights from single-cell and tissue exosome sequencing analysis Frontiers in immunology. 2026. DOI: 10.3389/fimmu.2026.1767771. PMID/PMCID: 42238572; PMC13226168.