患者血浆外泌体作为预测肺癌脑转移生物标志物的探索
核心信息
- 论文英文标题: Exploration of patient plasma exosomes as biomarkers for predicting lung cancer brain metastasis
- 期刊: FRONT MED
- 发表年份: 2025
- DOI: 10.3389/fmed.2025.1687866
- PMID/PMCID: 41416095; PMC12709389
研究概览
Many studies have found that exosomes have numerous advantages in the early diagnosis of tumors. Plasma exosomal miR-223-3p, miR-27a-3p, miR-27b-3p, and IBA, IVA, VA, and AA in advanced patients are closely associated with brain metastasis and have the potential to act as biomarkers for predicting brain metastasis in lung cancer patients.
核心发现
Exosomal miR-223-3p, miR-27a-3p, and miR-27b-3p were significantly increased in the plasma exosomes of lung cancer patients with brain metastasis.
The concentrations of isobutyric acid (IBA), valeric acid (VA), isovaleric acid (IVA), and acetic acid (AA) were markedly elevated in the plasma exosomes of lung cancer patients with brain metastasis.
Spearman correlation analysis revealed that both miR-27a-3p and miR-27b-3p had significant associations with IVA and VA.
研究设计
The total RNA of plasma exosomes of advanced lung cancer patients was extracted and sequenced.
The BLAST software was used to align the predicted target gene sequence against the GO and KEGG databases, thereby acquiring annotation details for the target genes.
The selected exosomal miRNAs and short-chain fatty acids were subjected to diagnostic performance validation analysis.
Echo Biotech 角色
血浆外泌体分离表征、miRNA测序分析+qPCR验证+短链脂肪酸代谢组
关联平台: Exoomics®, Research Reagents & Tools
关联服务与能力: Biofluid EV Isolation & Purification, EV Basic Characterization (TEM/NTA/WB), EV miRNA Profiling / Sequencing, EV Metabolomics/Lipidomics, qPCR Validation, Research Reagent / Product Supply
关联产品或试剂: Exosupur® EV Isolation/Purification Kit
参考文献
原始论文: Exploration of patient plasma exosomes as biomarkers for predicting lung cancer brain metastasis Frontiers in medicine. 2025. DOI: 10.3389/fmed.2025.1687866. PMID/PMCID: 41416095; PMC12709389.