脑源性外泌体miRNA的表达谱揭示了负责持续性大脑中动脉梗塞大鼠模型自发运动功能恢复的关键分子

核心信息

  • 论文英文标题: The expression profile of brain-derived exosomal miRNAs reveals the key molecules responsible for spontaneous motor function recovery in a rat model with permanent middle cerebral artery occlusion
  • 期刊: Mamm Genome
  • 发表年份: 2024
  • DOI: 10.1007/s00335-024-10052-5
  • PMID/PMCID: 38997467

研究概览

We assessed spontaneous motor function in a rat model of permanent middle cerebral artery occlusion (pMCAO) using motor function scores and magnetic resonance imaging (MRI). We assessed spontaneous motor function in a rat model of permanent middle cerebral artery occlusion (pMCAO) using motor function scores and magnetic resonance imaging (MRI).

核心发现

The analysis of alterations in the expression and functionality of brain-derived exosomal miRNAs within ischemic stroke lesions provides significant insights into the mechanisms that contribute to disease recovery.

We assessed spontaneous motor function in a rat model of permanent middle cerebral artery occlusion (pMCAO) using motor function scores and magnetic resonance imaging (MRI).

Brain-derived exosomes from the infarcted brain tissue of the animal model were extracted and high-throughput sequencing of them was performed followed by bioinformatics analysis for differentially expressed miRNAs target genes.

Real-time quantitative polymerase chain reaction (qRT-PCR) was used to measure expression levels of differentially expressed miRNAs at various time points.

Echo Biotech 角色

脑组织外泌体分离鉴定&miRNA测序分析&qPCR验证

关联平台: Exoomics®, Research Reagents & Tools

关联服务与能力: Tissue EV Isolation & Purification, EV Basic Characterization (TEM/NTA/WB), EV miRNA Profiling / Sequencing, qPCR Validation, Research Reagent / Product Supply

关联产品或试剂: Exosupur® EV Isolation/Purification Kit

参考文献

原始论文: The expression profile of brain-derived exosomal miRNAs reveals the key molecules responsible for spontaneous motor function recovery in a rat model with permanent middle cerebral artery occlusion Mammalian genome. 2024. DOI: 10.1007/s00335-024-10052-5. PMID/PMCID: 38997467.