通过工程化的晚期结构域支架在EVs表面显示αPD-L1和αCD3抗体以激活T细胞抗肿瘤免疫

核心信息

• 论文英文标题: Extracellular vesicle surface display of αPD-L1 and αCD3 antibodies via engineered late domain-based scaffold to activate T-cell anti-tumor immunity
• 期刊: J Extracell Vesicles
• 发表年份: 2024
• DOI: 10.1002/jev2.12490
• PMID/PMCID: 39051742; PMC11270581

研究概览

Inspired by the similarity of biogenesis between EVs and viruses, we developed a synthetic, Late domain-based EV scaffold protein that enables the display of a set of single chain variable fragments (scFvs) on the EV surface. Our study suggested that the LEAP scaffold may serve as a platform for EV surface display and could be applied for a broad range of EV-based biomedical applications.

核心发现

Extracellular vesicles (EVs) are emerging as promising carriers for the delivery of therapeutic biologics.

Genetic engineering represents a robust strategy for loading proteins of interest into EVs.

Identification of EV-enriched proteins facilitates protein cargo loading efficiency.

Many EV-enriched proteins are sorted into EVs via an endosomal sorting complex required for transport (ESCRT)-dependent pathway.

Echo Biotech 角色

Exosupur排阻柱ES914

关联平台: Exoomics®, Research Reagents & Tools

关联服务与能力: EV Isolation & Purification, Research Reagent / Product Supply

关联产品或试剂: Exosupur® EV Isolation/Purification Kit

参考文献

原始论文: Extracellular vesicle surface display of αPD-L1 and αCD3 antibodies via engineered late domain-based scaffold to activate T-cell anti-tumor immunity Journal of Extracellular Vesicles. 2024. DOI: 10.1002/jev2.12490. PMID/PMCID: 39051742; PMC11270581.