通过尺寸排阻色谱分离的细胞外囊泡适用于血浆中的 RNomics 分析

核心信息

• 论文英文标题: Extracellular vesicles isolated by size-exclusion chromatography present suitability for RNomics analysis in plasma
• 期刊: J Transl Med
• 发表年份: 2021
• DOI: 10.1186/s12967-021-02775-9
• PMID/PMCID: 33712033; PMC7953782

研究概览

Extracellular vesicles (EVs), known as cell-derived membranous structures harboring a variety of biomolecules, have been widely used in liquid biopsy. Due to the complex biological composition of plasma, plasma RNA omics analysis (RNomics) is easily affected, thus it is necessary to select an optimal strategy from exiting methods according to the performance for intended application.

核心发现

The results showed that the lowest miRNA binding protein AGO2 (Argonaute-2) and the highest EVs-specific miRNA and lncRNA were observed in EVs obtained through SEC, meanwhile the content of the non-specific miRNA was the lowest.

Further RNA-Seq data revealed that RNAs obtained via SEC presented more useful reads for both miRNA and mRNA.

Furthermore, the mRNA delivered via SEC tended to have a concentration comparable to the ideal FPKM (Fragments Per Kilobase Million) value.

研究设计

ultracentrifugation (UC), size exclusion chromatography (SEC), and two most frequently-used commercially available isolation kit (ExoQuick and exoEasy).

We compared the yield, purity, PCR quantification of RNAs, miRNA-seq analyses and mRNA-seq analyses of RNAs from EVs isolated using four methods.

Echo Biotech 角色

血浆外泌体全转录组测序分析

关联平台: Exoomics®, Research Reagents & Tools

关联服务与能力: Biofluid EV Isolation & Purification, EV Whole-Transcriptome Sequencing, Research Reagent / Product Supply

关联产品或试剂: Exosupur® EV Isolation/Purification Kit

参考文献

原始论文: Extracellular vesicles isolated by size-exclusion chromatography present suitability for RNomics analysis in plasma J Transl Med. 2021. DOI: 10.1186/s12967-021-02775-9. PMID/PMCID: 33712033; PMC7953782.