通过血浆外泌体转录组学鉴定调节局部晚期直肠癌化疗耐药性的Hsa-miR-483-5p/mRNA网络
核心信息
- 论文英文标题: Hsa-miR-483-5p/mRNA network that regulates chemotherapy resistance in locally advanced rectal cancer identified through plasma exosome transcriptomics
- 期刊: World J Clin Oncol
- 发表年份: 2024
- DOI: 10.5306/wjco.v15.i8.1061
- PMID/PMCID: 39193162; PMC11346070
研究概览
To detect the differential expression profiles of plasma exosomal microRNAs (miRNAs) in poor and good responders and explore the potential mechanisms of chemoresistance. However, the underlying mechanisms remain elusive.
核心发现
Exosome hsa-miR-483-5p was up-regulated in good responders post-nCRT.
Bioinformatics analysis revealed that the target genes of hsa-miR-483-5p were mainly enriched in tumor-specific pathways, such as the MAPK signaling pathway, EGFR tyrosine kinase inhibitor resistance, Toll-like receptor signaling pathway, VEGF signaling pathway, and mTOR signaling pathway.
Further analysis indicated that MAPK3, RAX2, and RNF165 were associated with inferior recurrence-free survival in patients with rectal cancer, and the profiles of MAPK3, TSPYL5, and ZNF417 were correlated with tumor stage.
In addition, the expression profiles of MAPK3, RNF165, and ZNF417 were negatively correlated with inhibitory concentration 50 values.
研究设计
In this study, the profiles of plasma exosomal miRNAs were compared in two dimensions according to treatment responses (poor/good responders) and treatment courses (pre/post-nCRT) using RNA sequencing.
Echo Biotech 角色
血浆外泌体分离鉴定&miRNA测序分析
关联平台: Exoomics®, Research Reagents & Tools
关联服务与能力: Biofluid EV Isolation & Purification, EV Basic Characterization (TEM/NTA/WB), EV miRNA Profiling / Sequencing, Research Reagent / Product Supply
关联产品或试剂: Exosupur® EV Isolation/Purification Kit
参考文献
原始论文: Hsa-miR-483-5p/mRNA network that regulates chemotherapy resistance in locally advanced rectal cancer identified through plasma exosome transcriptomics World journal of clinical oncology. 2024. DOI: 10.5306/wjco.v15.i8.1061. PMID/PMCID: 39193162; PMC11346070.