缺氧诱导的外泌体circ_0006840通过调节WIF1的降解促进胰腺癌的进展
核心信息
- 论文英文标题: Hypoxia-induced exosomal circ_0006840 promotes pancreatic cancer progress by regulating the WIF1 decay
- 期刊: BMC Biol
- 发表年份: 2026
- DOI: 10.1186/s12915-025-02320-w
- PMID/PMCID: 41484978; PMC12763943
研究概览
Quantitative reverse transcription PCR (RT-qPCR) and in situ hybridization (ISH) was used to assess the expression levels of circ_0006840 in PC patients. It has been shown that circ_0006840 was transcriptionally activated by HIF1A and specifically regulated WIF1 transcripts, which is considered a potential target for PC therapy.
核心发现
We identified differentially expressed circRNAs in exosomes derived from normoxic and hypoxic PC cells through RNA sequencing.
We show that high level of circ_0006840 was found in PC tissues and serum exosomes, which was associated with poor patient survival.
Both in vitro and in vivo, circ_0006840 enhanced PC cell proliferation and migration.
At the transcriptional level, HIF1A mediated circ_0006840 activation.
研究设计
A comparison of hypoxic versus normoxic PC cells was conducted using RNA sequencing to identify differentially expressed circRNAs.
Quantitative reverse transcription PCR (RT-qPCR) and in situ hybridization (ISH) was used to assess the expression levels of circ_0006840 in PC patients.
In vitro and in vivo experiments were conducted to validate the biological functions of circ_0006840 in PC.
Echo Biotech 角色
使用ExoLoad负载抑制circRNA的siRNA
关联平台: Echosome®, Research Reagents & Tools
关联服务与能力: Small RNA / Cargo Loading, Research Reagent / Product Supply
关联产品或试剂: ExoLoad® Nucleic Acid Loading Kit
参考文献
原始论文: Hypoxia-induced exosomal circ_0006840 promotes pancreatic cancer progress by regulating the WIF1 decay BMC biology. 2026. DOI: 10.1186/s12915-025-02320-w. PMID/PMCID: 41484978; PMC12763943.