低氧处理脂肪间充质干细胞来源外泌体增强对单侧输尿管梗阻小鼠的治疗作用

核心信息

• 论文英文标题: Hypoxia-treated adipose mesenchymal stem cells derived exosomes enhance the therapeutic effects on unilateral ureteral obstruction mice
• 期刊: Pharmacology
• 发表年份: 2025
• DOI: 10.1159/000542609
• PMID/PMCID: 39561719; PMC13200829

研究概览

The exosomes from adipose-derived mesenchymal stem cells (AMSCs) had therapeutic effects. The mechanism exploration revealed that exosomes could decrease the TNF-α and IL-6 and inhibit cell apoptosis.

核心发现

The cultured AMSCs could differentiate into osteoblast and adipocyte.

Meanwhile, the cultured AMSCs could effectively secrete the exosomes, which were characterized by around 110 nm diameter and surface marker expression.

Exosomes derived from hypoxia-treated AMSCs improved renal functions in UUO mice.

The mechanism exploration revealed that exosomes could decrease the TNF-α and IL-6 and inhibit cell apoptosis.

研究设计

The exosomes were characterized using a transmission electron microscope and Western blot.

Its size distribution was determined using the Zetasizer Nano ZS analysis system.

The differentiation ability was assessed by alkaline phosphatase and oil red staining.

Echo Biotech 角色

外泌体电镜

关联平台: Exoomics®

关联服务与能力: EV Basic Characterization (TEM/NTA/WB)

关联产品或试剂: 无明确产品引用

参考文献

原始论文: Hypoxia-treated adipose mesenchymal stem cells derived exosomes enhance the therapeutic effects on unilateral ureteral obstruction mice Pharmacology. 2025. DOI: 10.1159/000542609. PMID/PMCID: 39561719; PMC13200829.