循环血浆外泌体miRNA在绝经后骨质疏松症诊断中的鉴定和评价

核心信息

  • 论文英文标题: Identification and evaluation of circulating exosomal miRNAs for the diagnosis of postmenopausal osteoporosis
  • 期刊: J Orthop Surg Res
  • 发表年份: 2023
  • DOI: 10.1186/s13018-023-04020-z
  • PMID/PMCID: 37496029; PMC10373377

研究概览

In this study, we aim to identify PMOP-associated circulating exosomal miRNAs and evaluate their diagnostic performance. These results suggested that the plasma exosomal miRNAs had the potential to serve as noninvasive diagnostic biomarkers for PMOP.

核心发现

Twenty-seven miRNAs were identified as differentially expressed in PMOP versus controls in sequencing data, of which six exosomal miRNAs (miR-196-5p, miR-224-5p, miR320d, miR-34a-5p, miR-9-5p, and miR-98-5p) were confirmed to be differentially expressed in PMOP patients by qRT-PCR in the validation cohort.

In addition, the number of pregnancies was found to be an independent risk factor that can improve the performance of exosomal miRNAs in diagnosing PMOP.

研究设计

We performed next-generation sequencing and bioinformatics analysis of plasma exosomal miRNAs from 12 PMOP patients and 12 non-osteoporosis controls to identify PMOP-associated exosomal miRNAs, and then validated them in an independent natural community cohort with 26 PMOP patients and 21 non-osteoporosis controls.

Exosomes were isolated with the size exclusion chromatography method from the plasma of elder postmenopausal women.

The plasma exosomal miRNA profiles were characterized in PMOP paired with controls with next-generation sequencing.

Echo Biotech 角色

血浆外泌体分离鉴定及miRNA测序&qPCR验证

关联平台: Exoomics®, Research Reagents & Tools

关联服务与能力: Biofluid EV Isolation & Purification, EV Basic Characterization (TEM/NTA/WB), EV miRNA Profiling / Sequencing, qPCR Validation, Research Reagent / Product Supply

关联产品或试剂: Exosupur® EV Isolation/Purification Kit

参考文献

原始论文: Identification and evaluation of circulating exosomal miRNAs for the diagnosis of postmenopausal osteoporosis J Orthop Surg Res. 2023. DOI: 10.1186/s13018-023-04020-z. PMID/PMCID: 37496029; PMC10373377.