通过miRNA测序鉴定与术后乳腺癌患者蒽环类药物诱导肝损伤相关的血浆外泌体miRNA
核心信息
- 论文英文标题: Identification of exosomal miRNAs associated with the anthracycline-induced liver injury in postoperative breast cancer patients by small RNA sequencing
- 期刊: PeerJ
- 发表年份: 2020
- DOI: 10.7717/peerj.9021
- PMID/PMCID: 32355577; PMC7185038
研究概览
Seven post-chemotherapy patients were recruited in this study. Moreover, our study provided an experimental basis for experimental verification to reveal the actual function and mechanism of miRNAs in AILI.
核心发现
A total of 30 DE-miRNAs and 79 AILI-related target genes were identified.
AILI-related target genes of the DE-miRNAs are significantly enriched in NOD-like receptor signaling pathway, the HIF-1 signaling pathway, and the FoxO signaling pathway.
Then, the hub genes were screened and we discovered that IL-6 and SOD2 are the most critical genes that may be involved in the development of AILI through the activation of immune response and the occurrence of oxidative stress, respectively.
In addition, we found that miR-1-3p could potentially regulate most of the hub genes in the miRNA-hub gene network.
研究设计
Seven post-chemotherapy patients were recruited in this study.
After isolated plasma-derived exosomes, small RNA sequencing revealed exosomal miRNA profiles and differentially expressed miRNAs (DE-miRNAs) were identified between the liver injury group and non-liver injury group.
miRTarBase and miRDB were used to predict the potential target genes of DE-miRNAs.
Echo Biotech 角色
血浆外泌体分离鉴定&miRNA测序分析
关联平台: Exoomics®
关联服务与能力: Biofluid EV Isolation & Purification, EV Basic Characterization (TEM/NTA/WB), EV miRNA Profiling / Sequencing
关联产品或试剂: 无明确产品引用
参考文献
原始论文: Identification of exosomal miRNAs associated with the anthracycline-induced liver injury in postoperative breast cancer patients by small RNA sequencing PeerJ. 2020. DOI: 10.7717/peerj.9021. PMID/PMCID: 32355577; PMC7185038.