脓毒症急性肺损伤肺组织外泌体非编码RNA和mRNA表达谱的综合分析

核心信息

  • 论文英文标题: Integrated Analysis of Non-Coding RNA and mRNA Expression Profiles in Exosomes from Lung Tissue with Sepsis-Induced Acute Lung Injury
  • 期刊: J Inflamm Res
  • 发表年份: 2023
  • DOI: 10.2147/JIR.S419491
  • PMID/PMCID: 37674532; PMC10478974

研究概览

The purpose of this study was to investigate the expression profile and related networks of long noncoding RNAs (lncRNAs), microRNAs (miRNAs), and mRNAs in lung tissue exosomes obtained from sepsis-induced ALI. The purpose of this study was to investigate the expression profile and related networks of long noncoding RNAs (lncRNAs), microRNAs (miRNAs), and mRNAs in lung tissue exosomes obtained from sepsis-induced ALI.

核心发现

Thirty lncRNA-miRNA-mRNA interactions were identified, including two upregulated lncRNAs, 30 upregulated miRNAs, and two downregulated miRNAs.

Based on the expression levels of differentially expressed mRNAs(DEmRNAs), differentially expressed LncRNAs(DELncRNAs), and differentially expressed miRNAs(DEmiRNAs), 30 ceRNA networks were constructed.

研究设计

A mouse model of sepsis was established using the cecal ligation and puncture method.

RNA sequencing was performed using lung tissue exosomes obtained from mice in the sham and CLP groups.

Hematoxylin-eosin staining, Western blotting, immunofluorescence, quantitative real-time polymerase chain reaction, and nanoparticle tracking analysis were performed to identify relevant phenotypes, and bioinformatic algorithms were used to evaluate competitive endogenous RNA (ceRNA) networks.

Echo Biotech 角色

肺组织外泌体分离鉴定&全转录组测序分析

关联平台: Exoomics®, Research Reagents & Tools

关联服务与能力: Tissue EV Isolation & Purification, EV Basic Characterization (TEM/NTA/WB), EV Whole-Transcriptome Sequencing, Research Reagent / Product Supply

关联产品或试剂: Exosupur® EV Isolation/Purification Kit

参考文献

原始论文: Integrated Analysis of Non-Coding RNA and mRNA Expression Profiles in Exosomes from Lung Tissue with Sepsis-Induced Acute Lung Injury J Inflamm Res. 2023. DOI: 10.2147/JIR.S419491. PMID/PMCID: 37674532; PMC10478974.