软骨组织EVs miRNA与膝骨关节炎单细胞RNA-seq图谱的综合分析

核心信息

• 论文英文标题: Integrative analysis of miRNA in cartilage-derived extracellular vesicles and single-cell RNA-seq profiles in knee osteoarthritis
• 期刊: Arch Biochem Biophys
• 发表年份: 2023
• DOI: 10.1016/j.abb.2023.109785
• PMID/PMCID: 37844826

研究概览

Extracellular vesicular miRNAs (EV-miRNAs) play essential roles as intercellular communication molecules in knee Osteoarthritis (OA). Our results provided some new insights into cartilage injury and knee OA pathogenesis which could improve the new diagnosis and treatment methods for OA.

核心发现

Extracellular vesicular miRNAs (EV-miRNAs) play essential roles as intercellular communication molecules in knee Osteoarthritis (OA).

We isolated cartilage-derived extracellular vesicles (EVs), to perform miRNA sequencing, which revealed EV-miRNA profiles and identified differentially expressed miRNAs (DE-miRNAs) between cartilage injury and cartilage non-injury groups.

The target genes of known and novel DE-miRNAs were predicted with multiMiR package in 14 miRNA-target interaction databases.

Meanwhile, single-cell RNA sequencing (scRNA-seq) was performed to identify chondrocyte clusters and their gene signatures in knee OA.

Echo Biotech 角色

软骨组织外泌体分离鉴定及miRNA测序分析

关联平台: Exoomics®, Research Reagents & Tools

关联服务与能力: Tissue EV Isolation & Purification, EV Basic Characterization (TEM/NTA/WB), EV miRNA Profiling / Sequencing, Research Reagent / Product Supply

关联产品或试剂: Exosupur® EV Isolation/Purification Kit

参考文献

原始论文: Integrative analysis of miRNA in cartilage-derived extracellular vesicles and single-cell RNA-seq profiles in knee osteoarthritis Arch Biochem Biophys. 2023. DOI: 10.1016/j.abb.2023.109785. PMID/PMCID: 37844826.