肾脏组织富含整合素β1蛋白的EVs募集Fn1+巨噬细胞加重缺血再灌注诱导的炎症
核心信息
- 论文英文标题: Integrin β1-rich extracellular vesicles of kidney recruit Fn1+ macrophages to aggravate ischemia-reperfusion-induced inflammation
- 期刊: JCI Insight
- 发表年份: 2024
- DOI: 10.1172/jci.insight.169885
- PMID/PMCID: 38258908; PMC10906229
研究概览
Ischemia-reperfusion injury-induced (IRI-induced) acute kidney injury is accompanied by mononuclear phagocyte (MP) invasion and inflammation. Our findings establish the molecular bases by which chemokines and kidney-EV mediate PT-MP crosstalk in early IRI and provide insights into systematic intercellular communication.
核心发现
Ischemia-reperfusion injury-induced (IRI-induced) acute kidney injury is accompanied by mononuclear phagocyte (MP) invasion and inflammation.
However, systematic analysis of extracellular vesicle-carried (EV-carried) proteins mediating intercellular crosstalk in the IRI microenvironment is still lacking.
Multiomics analysis combining single-cell RNA-Seq data of kidney and protein profiling of kidney-EV was used to elucidate the intercellular communication between proximal tubular cells (PTs) and MP.
Targeted adhesion and migration of various MPs were caused by the secretion of multiple chemokines as well as integrin β1-rich EV by ischemic-damaged PTs after IRI.
Echo Biotech 角色
肾脏组织外泌体分离鉴定及蛋白质组 及 组织外泌体蛋白质组与单细胞转录组联合分析（含贝瑞单细胞转录组数据的重新分析成文）
关联平台: Exoomics®, Research Reagents & Tools
关联服务与能力: Tissue EV Isolation & Purification, EV Basic Characterization (TEM/NTA/WB), EV Proteomics, Single-Cell RNA Sequencing, Integrated Multi-omics Analysis, Research Reagent / Product Supply
关联产品或试剂: Exosupur® EV Isolation/Purification Kit
参考文献
原始论文: Integrin β1-rich extracellular vesicles of kidney recruit Fn1+ macrophages to aggravate ischemia-reperfusion-induced inflammation JCI Insight. 2024. DOI: 10.1172/jci.insight.169885. PMID/PMCID: 38258908; PMC10906229.