分离和富集双膜蛋白阳性EVs用于后续生物学研究
核心信息
- 论文英文标题: Isolation and enrichment of extracellular vesicles with double-positive membrane protein for subsequent biological studies
- 期刊: Adv Healthc Mater
- 发表年份: 2023
- DOI: 10.1002/adhm.202303430
- PMID/PMCID: 37942845
研究概览
The isolation and enrichment of specific extracellular vesicle (EV) subpopulations are essential in the context of precision medicine. However, the current methods predominantly rely on a single-positive marker and are susceptible to interference from soluble proteins or impurities.
核心发现
The isolation and enrichment of specific extracellular vesicle (EV) subpopulations are essential in the context of precision medicine.
However, the current methods predominantly rely on a single-positive marker and are susceptible to interference from soluble proteins or impurities.
This limitation represents a significant obstacle to the widespread application of EVs in biological research.
Herein, a novel approach that utilizes proximity ligation assay (PLA) and DNA-RNA hybridization are proposed to facilitate the binding of two proteins on the EV membrane in advance enabling the isolation and enrichment of intact EVs with double-positive membrane proteins followed by using functionalized magnetic beads for capture and enzymatic cleavage for isolated EVs release.
Echo Biotech 角色
外泌体miRNA测序分析
关联平台: Exoomics®
关联服务与能力: EV miRNA Profiling / Sequencing
关联产品或试剂: 无明确产品引用
参考文献
原始论文: Isolation and enrichment of extracellular vesicles with double-positive membrane protein for subsequent biological studies Adv Healthc Mater. 2023. DOI: 10.1002/adhm.202303430. PMID/PMCID: 37942845.