用于体内外泌体药代动力学定量分析的无标记液相色谱-串联质谱法
核心信息
- 论文英文标题: A Label-Free Liquid Chromatography–Tandem Mass Spectrometry Method for the Quantitative Analysis of Exosome Pharmacokinetics In Vivo
- 期刊: Pharmaceutics
- 发表年份: 2025
- DOI: 10.3390/pharmaceutics17060699
- PMID/PMCID: 40574012; PMC12195665
研究概览
Methods: To address this challenge, we developed a label-free liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to investigate the PK of naive exosome-based therapeutic modalities. Due to their complex and heterogeneous composition, most existing PK methods rely on chemical or genetic labeling, which may alter their native behavior and complicate accurate analysis.
核心发现
Background: Exosomes are nanoscale extracellular vesicles actively secreted by cells that play critical roles in disease biomarker discovery, drug delivery, and direct therapeutic applications.
However, in vivo pharmacokinetic (PK) studies of exosomes remain limited, hindering their clinical translation.
Due to their complex and heterogeneous composition, most existing PK methods rely on chemical or genetic labeling, which may alter their native behavior and complicate accurate analysis.
Methods: To address this challenge, we developed a label-free liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to investigate the PK of naive exosome-based therapeutic modalities.
Echo Biotech 角色
293F外泌体静脉注射大鼠检测血液中外泌体的药代动力学---引用产品:ContrExo 293F外泌体标准品和Exosupur微量血浆排阻柱分离大鼠血浆外泌体
关联平台: Exoomics®, EchoPharm®, EV Materials / Reference Materials, Research Reagents & Tools
关联服务与能力: Biofluid EV Isolation & Purification, EV Material / Reference Material Supply, EV Pharmacokinetics Support
关联产品或试剂: Exosupur® EV Isolation/Purification Kit, ContrExo® EV Reference Material
参考文献
原始论文: A Label-Free Liquid Chromatography–Tandem Mass Spectrometry Method for the Quantitative Analysis of Exosome Pharmacokinetics In Vivo Pharmaceutics. 2025. DOI: 10.3390/pharmaceutics17060699. PMID/PMCID: 40574012; PMC12195665.