龄龟丸通过调节精浆外泌体Wnt信号通路改善弱精症：基于LRP6/GSK3/Septin4通路的研究

核心信息

  • 论文英文标题: Linggui formula improves asthenozoospermia by modulating seminal plasma exosomes Wnt signaling via the LRP6/GSK3/Septin4 pathway
  • 期刊: J Ethnopharmacol
  • 发表年份: 2025
  • DOI: 10.1016/j.jep.2025.120495
  • PMID/PMCID: 40882792

研究概览

This study employs the kidney-tonifying Chinese herbal medicine Linggui formula (LG) as a representative to investigate its mechanism in treating asthenozoospermia (AS) through the modulation of sEVs. LG may enhance sperm motility by modulating Wnt proteins in the epididymis, testis, and prostate, thereby upregulating sEVs-mediated Wnt signaling via the LRP6/GSK3/Septin4 pathway.

核心发现

sEVs were successfully isolated and characterized, revealing that sperm retain the capacity to receive signals in vitro, allowing sEVs-mediated influence on their activity.

LG upregulated sEVs-mediated Wnt signaling, activating the phosphorylation of sperm LRP6, reducing GSK3 activity, and promoting Septin4 polymerization, thereby enhancing sperm motility.

In vivo studies demonstrated the presence of multiple Wnt ligands with distinct expression patterns across different locations within the reproductive system.

研究设计

In vitro, sEVs were isolated from AS patients, AS patients post-LG treatment, and healthy subjects, followed by analysis of differential Wnt protein expression.

Spermatozoa from AS patients were cultured with these sEVs to assess motility and uptake, and sperm p-LRP6, p-GSK3, and Septin4 expression after sEVs treatment was evaluated.

In vivo, the Wnt signaling effects of sEVs from different rat epididymal segments, testis, and prostate were compared to identify the therapeutic target of LG for AS.

Echo Biotech 角色

Exosupur ES933分离精浆外泌体

关联平台: Exoomics®, Research Reagents & Tools

关联服务与能力: Biofluid EV Isolation & Purification, Research Reagent / Product Supply

关联产品或试剂: Exosupur® EV Isolation/Purification Kit

参考文献

原始论文: Linggui formula improves asthenozoospermia by modulating seminal plasma exosomes Wnt signaling via the LRP6/GSK3/Septin4 pathway Journal of ethnopharmacology. 2025. DOI: 10.1016/j.jep.2025.120495. PMID/PMCID: 40882792.