LncRNA CASC15 在骨肉瘤血浆外泌体中上调，CASC15 敲低通过调节 miR-338-3p/RAB14 轴抑制骨肉瘤进展
核心信息
- 论文英文标题: LncRNA CASC15 is Upregulated in Osteosarcoma Plasma Exosomes and CASC15 Knockdown Inhibits Osteosarcoma Progression by Regulating miR-338-3p/RAB14 Axis
- 期刊: Onco Targets Ther
- 发表年份: 2020
- DOI: 10.2147/OTT.S282053
- PMID/PMCID: 33262606; PMC7700090
研究概览
The objective of this study is to reveal the dysregulated lncRNAs in OS plasma exosomes and explore their functions in OS. Further assays indicated that CASC15 knockdown could restrain the proliferation, migration, and invasion of OS cells, and inhibit the growth of OS in xenograft models.
核心发现
Upregulated CASC15 was observed in OS plasma exosomes compared with control, and the same expression was observed in the OS tissues and cell lines.
Further assays indicated that CASC15 knockdown could restrain the proliferation, migration, and invasion of OS cells, and inhibit the growth of OS in xenograft models.
Furthermore, our results demonstrated CASC15 regulated OS progression via acting as miR-338-3p sponge, and RAB14 was a direct downstream target of miR-338-3p.
Rescue experiments verified CASC15 promotes OS cell growth and metastasis by upregulating RAB14 expression.
研究设计
Microarray was performed to analyze dysregulated exosomal lncRNAs.
Western blot, qRT-PCR assays, and Dual-luciferase reporter assay were used to verify the interaction among cancer susceptibility 15 (CASC15), miR-338-3p, and RAB14.
Cck-8, colony formation assay, and transwell assay were performed to explore and characterize the effects of CASC15 on OS cells.
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关联平台: Exoomics®, Research Reagents & Tools
关联服务与能力: EV Isolation & Purification, Research Reagent / Product Supply
关联产品或试剂: Exosupur® EV Isolation/Purification Kit
参考文献
原始论文: LncRNA CASC15 is Upregulated in Osteosarcoma Plasma Exosomes and CASC15 Knockdown Inhibits Osteosarcoma Progression by Regulating miR-338-3p/RAB14 Axis Onco Targets Ther. 2020. DOI: 10.2147/OTT.S282053. PMID/PMCID: 33262606; PMC7700090.