LncRNA CCAT1通过PTBP1介导的糖酵解增强促进胃癌进展

核心信息

  • 论文英文标题: LncRNA CCAT1 facilitates the progression of gastric cancer via PTBP1-mediated glycolysis enhancement
  • 期刊: J EXP CLIN CANC RES
  • 发表年份: 2023
  • DOI: 10.1186/s13046-023-02827-6
  • PMID/PMCID: 37740243; PMC10517515

研究概览

Gain- and loss-of-function experiments were performed to evaluate the roles of CCAT1 in tumorigenesis and glycolysis of GC. Our findings demonstrate that CCAT1 plays a significant role in promoting the proliferation, migration, and invasion of GC cells through the PTBP1/PKM2/glycolysis pathway, thus suggesting CCAT1's potential as a biomarker and therapeutic target for GC.

核心发现

In this study, we identified that CCAT1 expression was significantly elevated in the tissues and plasma exosomes of GC patients, as well as GC cell lines.

Functional experiments showed that the knockdown of CCAT1 resulted in a substantial decrease in the proliferation, migration and invasion of GC cells both in vitro and in vivo through decreasing the expression of glycolytic enzymes and glycolytic rate.

Conversely, overexpression of CCAT1 exhibited contrasting effects.

Mechanistically, CCAT1 interacted with PTBP1 and effectively maintained its stability by inhibiting the ubiquitin-mediated degradation process.

研究设计

Gain- and loss-of-function experiments were performed to evaluate the roles of CCAT1 in tumorigenesis and glycolysis of GC.

Bioinformatics analyses and mechanistic experiments, such as mass spectrometry (MS), RNA-pulldown, and RNA immunoprecipitation (RIP), were employed to reveal the potential interacting protein of CCAT1 and elucidate the regulatory mechanism of CCAT1 in GC glycolysis.

Moreover, the nude mice xenograft assay was used to evaluate the effect of CCAT1 on GC cells in vivo.

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参考文献

原始论文: LncRNA CCAT1 facilitates the progression of gastric cancer via PTBP1-mediated glycolysis enhancement Journal of experimental & clinical cancer research. 2023. DOI: 10.1186/s13046-023-02827-6. PMID/PMCID: 37740243; PMC10517515.