骨折大鼠模型中巨噬细胞来源的外泌体lncRNA促进BMSC成骨
核心信息
- 论文英文标题: Long Non-Coding RNAs Within Macrophage-Derived Exosomes Promote BMSC Osteogenesis in a Bone Fracture Rat Model
- 期刊: INT J NANOMED
- 发表年份: 2023
- DOI: 10.2147/IJN.S398446
- PMID/PMCID: 36879890; PMC9985426
研究概览
To investigate the effect of macrophage exosomal long non-coding (lnc)RNAs on bone mesenchymal stem cell (BMSC) osteogenesis and the associated mechanism. Rat BMSCs and spleen macrophages were co-cultured with serum derived from the fracture microenvironment of rat tibia.
核心发现
In the fracture microenvironment, macrophages (stimulated using either hypoxia or CSF) significantly increased the osteogenic ability of BMSCs.
We showed that BMSCs assimilated macrophage-derived vesicles and that the inhibition of exosomal secretion significantly attenuated the macrophage-mediated induction of BMSC osteogenesis.
The hypoxia condition led to the up-regulation of 310 lncRNAs and the down-regulation of 575 lncRNAs in macrophage exosomes, while CSF stimulation caused the up-regulation of 557 lncRNAs and the down-regulation of 407 lncRNAs.
In total, 108 lncRNAs were co-up-regulated and 326 lncRNAs were co-down-regulated under both conditions.
研究设计
Rat BMSCs and spleen macrophages were co-cultured with serum derived from the fracture microenvironment of rat tibia.
BMSC osteogenesis was evaluated using Alizarin red staining and the expression of BMP-2, RUNX2, OPN, and OC mRNA.
BMSC osteogenesis was evaluated after co-culture with macrophages stimulated using hypoxic conditions or colony-stimulating factor (CSF).
Echo Biotech 角色
大鼠巨噬细胞外泌体长链RNA测序分析
关联平台: Exoomics®
关联服务与能力: EV lncRNA/mRNA Sequencing
关联产品或试剂: 无明确产品引用
参考文献
原始论文: Long Non-Coding RNAs Within Macrophage-Derived Exosomes Promote BMSC Osteogenesis in a Bone Fracture Rat Model International journal of nanomedicine. 2023. DOI: 10.2147/IJN.S398446. PMID/PMCID: 36879890; PMC9985426.