巨噬细胞微囊泡miR-350-3p通过下调软骨细胞H3K36甲基转移酶NSD1促进骨关节炎进展

核心信息

• 论文英文标题: Macrophage-derived ectosomal miR-350-3p promotes osteoarthritis progression through downregulating chondrocyte H3K36 methyltransferase NSD1
• 期刊: Cell Death Discov
• 发表年份: 2024
• DOI: 10.1038/s41420-024-01986-5
• PMID/PMCID: 38719811; PMC11078928

研究概览

Here, we found that macrophages exhibited M1 polarization under mechanical overloading and secreted ectosomes that induced cartilage degradation and senescence. This study demonstrated that the targeting of macrophage-derived ectosomal miRNAs was a potential therapeutic method for mechanical overload-induced OA.

核心发现

Mechanical overloading can promote cartilage senescence and osteoarthritis (OA) development, but its impact on synovial macrophages and the interaction between macrophages and chondrocytes remain unknown.

Here, we found that macrophages exhibited M1 polarization under mechanical overloading and secreted ectosomes that induced cartilage degradation and senescence.

By performing miRNA sequencing on ectosomes, we identified highly expressed miR-350-3p as a key factor mediating the homeostatic imbalance of chondrocytes caused by M1-polarized macrophages, this result being confirmed by altering the miR-350-3p level in chondrocytes with mimics and inhibitor.

In experimental OA mice, miR-350-3p was increased in synovium and cartilage, while intra-articular injection of antagomir-350-3p inhibited the increase of miR-350-3p and alleviated cartilage degeneration and senescence.

Echo Biotech 角色

微囊泡分离鉴定&miRNA测序分析

关联平台: Exoomics®

关联服务与能力: EV Isolation & Purification, EV Basic Characterization (TEM/NTA/WB), EV miRNA Profiling / Sequencing

关联产品或试剂: 无明确产品引用

参考文献

原始论文: Macrophage-derived ectosomal miR-350-3p promotes osteoarthritis progression through downregulating chondrocyte H3K36 methyltransferase NSD1 Cell Death Discov. 2024. DOI: 10.1038/s41420-024-01986-5. PMID/PMCID: 38719811; PMC11078928.