褪黑素预处理的间充质干细胞外泌体通过miR-145-5pTGF-βSmad轴缓解神经损伤诱导的勃起功能障碍大鼠模型中的海绵体纤维化
核心信息
- 论文英文标题: Melatonin-pretreated mesenchymal stem cell-derived exosomes alleviate cavernous fibrosis in a rat model of nerve injury-induced erectile dysfunction via miR-145-5p/TGF-β/Smad axis
- 期刊: Stem Cell Res Ther
- 发表年份: 2025
- DOI: 10.1186/s13287-025-04173-0
- PMID/PMCID: 40001250; PMC11863846
研究概览
Cavernous nerve injury-induced erectile dysfunction (CNI-ED) is a common complication after radical prostatectomy. Conventional treatment approaches have had little success in treating the severe cavernous fibrosis which is a consequence of CNI-ED.
核心发现
Intracavernous injection of MT-hASC-EVs significantly restored erectile function and reduced the extent of corpus cavernosum fibrosis in the CNI-ED rat model.
MT-hASC-EVs promoted the proliferation and anti-apoptotic effects of corpus cavernosum smooth muscle cells (CCSMCs) in vitro.
Mechanistically, MT-hASC-EVs inhibit fibrosis by delivering miR-145-5p, which targets TGF-β2/Smad3 axis.
研究设计
Pre-treatment of adipose-derived stem cells with melatonin allows for the extraction of active exosomes (MT-hASC-EVs) from the conditioned medium.
The therapeutic effects of MT-hASC-EVs were assessed in a rat model of CNI-ED, and the anti-fibrotic properties were evaluated.
MicroRNA sequencing was used to identify specific microRNAs highly expressed in MT-hASC-EVs, and differential microRNAs were screened for regulatory pathways through target gene enrichment analysis.
Echo Biotech 角色
ExoLoad试剂盒
关联平台: Echosome®, Research Reagents & Tools
关联服务与能力: Small RNA / Cargo Loading, Research Reagent / Product Supply
关联产品或试剂: ExoLoad® Nucleic Acid Loading Kit
参考文献
原始论文: Melatonin-pretreated mesenchymal stem cell-derived exosomes alleviate cavernous fibrosis in a rat model of nerve injury-induced erectile dysfunction via miR-145-5p/TGF-β/Smad axis Stem cell research & therapy. 2025. DOI: 10.1186/s13287-025-04173-0. PMID/PMCID: 40001250; PMC11863846.