肠系膜脂肪组织外泌体TINAGL1通过SMAD4增强克罗恩病肠纤维化
核心信息
- 论文英文标题: Mesenteric adipose-derived exosomal TINAGL1 enhances intestinal fibrosis in Crohn's Disease via SMAD4
- 期刊: J Adv Res
- 发表年份: 2024
- DOI: 10.1016/j.jare.2024.05.016
- PMID/PMCID: 38750695; PMC11976418
研究概览
This study aims to elucidate the role of MAT-derived exosomes in CD-related intestinal fibrosis. The involvement of the TGF-β signaling pathway, especially the SMAD4 protein, offers new insights into the molecular mechanisms of CD-related fibrosis and presents potential targets for therapeutic intervention.
核心发现
Proteomic analyses revealed a significant activation of the TGF-β signaling pathway in MG-treated mice compared to controls, correlating with enhanced intestinal fibrosis.
In vitro experiments demonstrated that colonic fibroblasts exposed to CD patient-derived exosomes exhibited increased fibrogenic activity.
Protein docking and co-immunoprecipitation studies suggested a critical interaction between TINAGL1 and SMAD4, enhancing fibrosis.
Importantly, in vivo experiments corroborated that recombinant TINAGL1 protein exacerbated DNBS-induced intestinal fibrosis.
研究设计
We induced chronic intestinal inflammation in mice using dinitrobenzene sulfonic acid (DNBS), simulating CD-like fibrosis.
Exosomes were isolated from DNBS-treated mice (MG) and normal controls (NG) for characterization using electron microscopy and proteomic analysis.
Additionally, human colonic fibroblasts were exposed to exosomes from CD patients and healthy individuals, with subsequent assessment of fibrogenesis through proteomic and RNA sequencing analyses.
Echo Biotech 角色
肠系膜组织外泌体分离鉴定&LFQ蛋白质组
关联平台: Exoomics®
关联服务与能力: Tissue EV Isolation & Purification, EV Basic Characterization (TEM/NTA/WB), EV Proteomics
关联产品或试剂: 无明确产品引用
参考文献
原始论文: Mesenteric adipose-derived exosomal TINAGL1 enhances intestinal fibrosis in Crohn's Disease via SMAD4 J Adv Res. 2024. DOI: 10.1016/j.jare.2024.05.016. PMID/PMCID: 38750695; PMC11976418.