精浆细胞外囊泡转运的miRNA-222靶向BCL2L11抑制精子凋亡

核心信息

• 论文英文标题: MicroRNA-222 Transferred From Semen Extracellular Vesicles Inhibits Sperm Apoptosis by Targeting BCL2L11
• 期刊: Front Cell Dev Biol
• 发表年份: 2021
• DOI: 10.3389/fcell.2021.736864
• PMID/PMCID: 34820370; PMC8607813

研究概览

The study of the whole transcriptomes and proteomes of EVs in boar semen revealed some miRNAs may play an important role in these EVs interactions with Duroc sperm, and the findings suggest that the release of miR-222 by semen EVs is an important mechanism by which sperm viability is maintained and sperm apoptosis is reduced. Our studies provide a new insight of miR-222 in EVs regulation for sperm motility and sperm apoptosis.

核心发现

Seminal plasma contains a large number of extracellular vesicles (EVs).

However, the roles of these EVs and their interactions with sperm are not clear.

To identify the important molecules affecting sperm motility in EVs, we analyzed RNA from seminal plasma EVs of boars with different sperm motility using whole-transcriptome sequencing and proteomic analysis.

In total, 7 miRNAs, 67 lncRNAs, 126 mRNAs and 76 proteins were differentially expressed between the two groups.

Echo Biotech 角色

猪精浆外泌体NTA和电镜鉴定及全转录组测序分析

关联平台: Exoomics®

关联服务与能力: EV Basic Characterization (TEM/NTA/WB), EV Whole-Transcriptome Sequencing

关联产品或试剂: 无明确产品引用

参考文献

原始论文: MicroRNA-222 Transferred From Semen Extracellular Vesicles Inhibits Sperm Apoptosis by Targeting BCL2L11 Front Cell Dev Biol. 2021. DOI: 10.3389/fcell.2021.736864. PMID/PMCID: 34820370; PMC8607813.