脂肪MSC外泌体miR-125b-5p通过Keap1-Nrf2-GPX4减轻脓毒症肺损伤中的肺微血管内皮细胞铁死亡

核心信息

• 论文英文标题: miR-125b-5p in adipose derived stem cells exosome alleviates pulmonary microvascular endothelial cells ferroptosis via Keap1/Nrf2/GPX4 in sepsis lung injury
• 期刊: Redox Biol
• 发表年份: 2023
• DOI: 10.1016/j.redox.2023.102655
• PMID/PMCID: 36913799; PMC10023991

研究概览

This study is meant to explore the protective effect and mechanism of ADSCs exosomes on excessive inflammation PMVECs injury. Collectively, we illustrated a novel potentially therapeutic mechanism that miR-125b-5p in ADSCs exosomes could alleviate the inflammation induced PMVECs ferroptosis in sepsis induced acute lung injury via regulating Keap1/Nrf2/GPX4 expression, hence improve the acute lung injury in sepsis.

核心发现

We successfully isolated ADSCs exosomes, the characteristic of which were confirmed.

ADSCs exosomes reduced excessive inflammatory response induced ROS accumulation and cell injury in PMVECs.

Besides, ADSCs exosomes inhibited excessive inflammatory response induced ferroptosis while upregulated expression of GPX4 in PMVECs.

And further GPX4 inhibition experiments revealed that ADSCs exosomes alleviated inflammatory response induced ferroptosis via upregulating GPX4.

Echo Biotech 角色

Exoload试剂盒

关联平台: Echosome®, Research Reagents & Tools

关联服务与能力: Small RNA / Cargo Loading, Research Reagent / Product Supply

关联产品或试剂: ExoLoad® Nucleic Acid Loading Kit

参考文献

原始论文: miR-125b-5p in adipose derived stem cells exosome alleviates pulmonary microvascular endothelial cells ferroptosis via Keap1/Nrf2/GPX4 in sepsis lung injury Redox Biol. 2023. DOI: 10.1016/j.redox.2023.102655. PMID/PMCID: 36913799; PMC10023991.