脓毒症患者中性粒细胞EVs中miR-150-5p与脓毒性心肌病相关

核心信息

  • 论文英文标题: miR-150-5p in neutrophil-derived extracellular vesicles associated with sepsis-induced cardiomyopathy in septic patients
  • 期刊: Cell Death Discov
  • 发表年份: 2023
  • DOI: 10.1038/s41420-023-01328-x
  • PMID/PMCID: 36681676; PMC9867758

研究概览

The present study focused on the in-depth miRNA expression profiles of neutrophil-derived extracellular vesicles and explored the potential molecular biomarkers during the process of SIC. miR-150-5p may be a predictor of sepsis severity development but warrants further study.

核心发现

Sixty-eight miRNAs from neutrophil-derived extracellular vesicles were changed significantly between healthy controls and without septic cardiomyopathy patients (61 miRNAs upregulated and seven downregulated).

Thirty-eight miRNAs were differentially expressed in the septic cardiomyopathy patients.

27 common differentially expressed miRNAs were found in both groups with similar kinetics (23 miRNAs upregulated and four downregulated).

The enriched cellular signaling pathway mediated by miRNAs from sepsis to septic cardiomyopathy was the HIF-1 signaling system modulated septic inflammation.

研究设计

Neutrophil-derived extracellular vesicles were isolated from the blood samples in three sepsis patients with or without cardiomyopathy on day 1 and day 3 after ICU admission in comparison with three healthy controls.

miRNAs were determined by RNA sequencing.

The closely related differentially expressed miRNAs with SIC were further validated through qRT-PCR in the other cohorts of sepsis patients with (30 patients) or without cardiomyopathy (20 patients) and the association between miRNAs and the occurrence or disease severity of septic cardiomyopathy were stratified with logistic regression analysis.

Echo Biotech 角色

外泌体miRNA测序验证和外泌体鉴定

关联平台: Exoomics®

关联服务与能力: EV Basic Characterization (TEM/NTA/WB), EV miRNA Profiling / Sequencing

关联产品或试剂: 无明确产品引用

参考文献

原始论文: miR-150-5p in neutrophil-derived extracellular vesicles associated with sepsis-induced cardiomyopathy in septic patients Cell Death Discov. 2023. DOI: 10.1038/s41420-023-01328-x. PMID/PMCID: 36681676; PMC9867758.