复发性植入失败患者子宫内膜细胞外囊中的Mir-218-5p会损害植入前胚胎发育

核心信息

• 论文英文标题: Mir-218-5p from Extracellular Vesicles of Endometrium in Patients with Recurrent Implantation Failure Impairs Pre-Implantation Embryo Development
• 期刊: Int J Nanomedicine
• 发表年份: 2025
• DOI: 10.2147/IJN.S508491
• PMID/PMCID: 40331233; PMC12052006

研究概览

In this study, we aimed to investigate the effectiveness of miR-218-5p as a molecular factor in RIF-EVs. This research provides a novel and non-invasive insight into potential therapeutic strategies for RIF.

核心发现

Essential transcription factors for TE and ICM, such as Cdx2, Yap1, Sox2, Nanog, Tead4, were reduced at the mRNA level in the miR-218-5p treated morula.

001) and disruption of co-localization of Yap1 and Cdx2.

研究设计

The percentage of blastocyst development and hatching rates, embryo morphology, and the total cell number were measured.

RNA-sequencing was used to analyze transcriptional changes in embryos post miR-218-5p agomir treatment.

The abnormal segregation genes of trophectoderm (TE) and inner cell mass (ICM) were visualized via qRT-PCR and immunofluorescence staining.

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参考文献

原始论文: Mir-218-5p from Extracellular Vesicles of Endometrium in Patients with Recurrent Implantation Failure Impairs Pre-Implantation Embryo Development International journal of nanomedicine. 2025. DOI: 10.2147/IJN.S508491. PMID/PMCID: 40331233; PMC12052006.