MiR-3960通过靶向DEXI抑制膀胱癌进展

核心信息

• 论文英文标题: MiR-3960 inhibits bladder cancer progression via targeting of DEXI
• 期刊: BIOCHEM BIOPH RES CO
• 发表年份: 2023
• DOI: 10.1016/j.bbrc.2023.05.055
• PMID/PMCID: 37230046

研究概览

MicroRNAs (miRNAs) are dominant cargo in exosomes and act as master regulators of cell function, inhibiting mRNA translation and affecting gene silencing. Our results demonstrate the potential role of miR-3960-mediated inhibition of DEXI as a therapeutic strategy against BC.

核心发现

The results showed that miR-3960 levels in BC tissue were positively correlated with patient survival time.

Dexi was a major target of miR-3960.

Dexi knockout inhibited MB49 cell proliferation and promoted cisplatin- and gemcitabine-induced apoptosis.

Transfection of miR-3960 mimic inhibited DEXI expression and organoid growth.

研究设计

A microarray was used to identify miRNAs in mouse bladder carcinoma cell line MB49 exosomes.

Real-time reverse transcription polymerase chain reaction was used to examine the expression of miRNAs in BC and healthy donor serum.

Western blotting and immunohistochemical staining were used to examine the expression of dexamethasone-induced protein (DEXI) in patients with BC.

Echo Biotech 角色

根据描述及合同情况判断应该是exoload试剂盒，客户在文章中写错了

关联平台: Echosome®, Research Reagents & Tools

关联服务与能力: Small RNA / Cargo Loading, Research Reagent / Product Supply

关联产品或试剂: ExoLoad® Nucleic Acid Loading Kit

参考文献

原始论文: MiR-3960 inhibits bladder cancer progression via targeting of DEXI Biochemical and biophysical research communications. 2023. DOI: 10.1016/j.bbrc.2023.05.055. PMID/PMCID: 37230046.