富含miR-92a-1-5p的前列腺癌细胞外囊泡通过MAPK1和FoxO1调节破骨细胞功能
核心信息
- 论文英文标题: miR-92a-1-5p enriched prostate cancer extracellular vesicles regulate osteoclast function via MAPK1 and FoxO1
- 期刊: J EXP CLIN CANC RES
- 发表年份: 2023
- DOI: 10.1186/s13046-023-02685-2
- PMID/PMCID: 37131239; PMC10152631
研究概览
In the present study, we focused on engineering miR-92a-1-5p into EVs and determining any therapeutic roles and mechanisms of the engineered EVs. These experiments suggest that miR-92a-1-5p enriched EVs regulate osteoclast function via reduction of MAPK1 and FoxO1.
核心发现
Stable overexpression cells of miRNA-92a-5p was associated with EVs upregulating this microRNA, as confirmed by qPCR.
Further, miR-92a-1-5p enriched EVs promote osteoclast differentiation in vitro by reducing MAPK1 and FoxO1 expression, associated with increased osteoclast function as shown by TRAP staining and mRNA expression of osteoclast functional genes.
siRNA targeting MAPK1 or FoxO1 resulted in similar increase in osteoclast function.
injection promote osteolysis, which was associated with reduction of MAPK1 and FoxO1 expression in bone marrow.
研究设计
A stable prostate cancer cell line (MDA PCa 2b) overexpressing miR-92a-1-5p was constructed by lentivirus, and EVs were isolated by ultracentrifugation.
The overexpression of miR-92a-1-5p in both cells and EVs was tested using qPCR.
Osteoclast function was evaluated by Trap staining, mRNA expression of osteoclastic markers ctsk and trap, immunolabeling of CTSK and TRAP and microCT using either in vitro and in vivo assays.
Echo Biotech 角色
NTA检测
关联平台: Exoomics®
关联服务与能力: EV Basic Characterization (TEM/NTA/WB)
关联产品或试剂: 无明确产品引用
参考文献
原始论文: miR-92a-1-5p enriched prostate cancer extracellular vesicles regulate osteoclast function via MAPK1 and FoxO1 Journal of experimental & clinical cancer research. 2023. DOI: 10.1186/s13046-023-02685-2. PMID/PMCID: 37131239; PMC10152631.